Thomas Roger C, Bers Donald M
Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3EG, United Kingdom.
Cold Spring Harb Protoc. 2013 Apr 1;2013(4):370-3. doi: 10.1101/pdb.prot072850.
In this protocol we describe how to make and use minielectrodes for measuring [Ca(2+)] in small volumes of solution. The minielectrodes are 2 mm in diameter and have sufficiently low resistances to be used with a standard pH meter. They are made by dipping polyethylene or borosilicate glass tubes (5 cm long) in a membrane solution. Although the chemicals used to make these Ca(2+)-sensitive minielectrodes are expensive, they can be used to make hundreds of electrodes, each with a useful life of several months.
在本方案中，我们描述了如何制作和使用微型电极来测量小体积溶液中的[Ca(2+)]。微型电极的直径约为2毫米，电阻足够低，可与标准pH计配合使用。它们是通过将聚乙烯或硼硅酸盐玻璃管（约5厘米长）浸入膜溶液中制成的。虽然用于制作这些Ca(2+)敏感微型电极的化学物质很昂贵，但它们可以用来制作数百个电极，每个电极的使用寿命为几个月。
