Flinders Centre for Nanoscale Science and Technology, Flinders University, GPO Box 2100, Adelaide, S.A, 5001 Australia.
J Am Chem Soc. 2013 Apr 17;135(15):5612-9. doi: 10.1021/ja310991r. Epub 2013 Apr 3.
Toehold-mediated DNA strand displacement provides unique advantages in the construction and manipulation of multidimensional DNA nanostructures as well as nucleic acid sequence analysis. We demonstrate a step change in the use of toehold-mediated DNA strand displacement reactions, where a double-stranded DNA duplex, containing a single-stranded toehold domain, enzymatically generated and then treated as a molecular target for analysis. The approach was successfully implemented for human DNA genotyping, such as gender identification where the amelogenin gene was used as a model target system, and detecting single nucleotide polymorphisms of human mitochondrial DNA. Kinetics of the strand displacement was monitored by the quenched Förster resonance energy transfer effect.
适体介导的 DNA 链置换在多维 DNA 纳米结构的构建和操作以及核酸序列分析中提供了独特的优势。我们展示了适体介导的 DNA 链置换反应的使用的重大改进，其中双链 DNA 双链体包含单链适体结构域，该结构域通过酶促反应生成，然后作为分析的分子靶标进行处理。该方法成功地用于人类 DNA 基因分型，例如使用 amelogenin 基因作为模型靶系统的性别鉴定，以及检测人类线粒体 DNA 的单核苷酸多态性。通过猝灭的Förster 共振能量转移效应来监测链置换的动力学。
