Mainil-Varlet Pierre, Schiavinato Antonella, Ganster Murielle Marie
Aginko Research AG, Marly, Switzerland.
Cartilage. 2013 Jan;4(1):28-41. doi: 10.1177/1947603512455193.
: To test the efficacy of a hyaluronan derivative (HYADD4-G) in a model of osteoarthritis (anterior cruciate ligament [ACLT]) and to compare its efficacy with the injection of growth factors. : In a first experimental set-up, specially selected for treatment scheme with published studies on hyaluronan or growth factor efficacy in osteoarthritis, saline, HYADD4-G, rh-BMP-7, and the treatments of rh-BMP-7 or rh-BMP-2 with HYADD4-G were injected after ACLT, for five times starting 3 weeks after ACLT. Euthanasia was at day 70. The knees were evaluated by gross morphological observation, x-ray, and histology (Study A). In a second experimental set-up selected to evaluate the efficacy of three viscosupplement injections, starting 4 weeks after ACTL, HYADD4-G was compared to saline (Study B). : (A) X-ray analysis showed more damage in the saline group than all other treatment groups (2.67 ± 0.61 for saline, 0.83 ± 0.26 for HYADD4-G, 1.67 ± 0.82 for HYADD4-G with rh-BMP-2, 0.75 ± 0.76 for HYADD4-G with rh-BMP-7, and 1.58 ± 0.49 for rh-BMP-7), < 0.05. In the femoral condyle, the Mankin's score for HYADD4-G with rh-BMP-2, HYADD4-G with rh-BMP-7, and rh-BMP7 alone was statistically lower compared to saline in the medial part; in the lateral part a significant lower value was observed in the HYADD4-G with the rh-BMP-2 group. (B) The Kellgren and Lawrence score and Mankin's score was lower in the HYADD4-G group than in the saline group ( < 0.002 and = 0.0031). : These two studies suggest that HYADD4-G delayed the cartilage degeneration and that the association of HYADD4-G with growth factors is synergistic.
为了在骨关节炎模型（前交叉韧带切断术[ACLT]）中测试一种透明质酸衍生物（HYADD4-G）的疗效，并将其疗效与生长因子注射进行比较。：在第一个实验设置中，根据已发表的关于透明质酸或生长因子在骨关节炎中疗效的研究，特别选择治疗方案，在ACLT后注射生理盐水、HYADD4-G、重组人骨形态发生蛋白-7（rh-BMP-7），以及rh-BMP-7或rh-BMP-2与HYADD4-G的联合治疗，从ACLT后3周开始，共注射5次。在第70天实施安乐死。通过大体形态观察、X射线和组织学对膝关节进行评估（研究A）。在第二个实验设置中，为了评估三次关节腔内注射透明质酸类药物的疗效，从ACLT后4周开始，将HYADD4-G与生理盐水进行比较（研究B）。：（A）X射线分析显示，生理盐水组的损伤比所有其他治疗组都更严重（生理盐水组为2.67±0.61，HYADD4-G组为0.83±0.26，HYADD4-G与rh-BMP-2联合治疗组为1.67±0.82，HYADD4-G与rh-BMP-7联合治疗组为0.75±0.76，rh-BMP-7组为1.58±0.49），P<0.05。在股骨髁中，与生理盐水组相比，HYADD4-G与rh-BMP-2联合治疗组、HYADD4-G与rh-BMP-7联合治疗组以及单独使用rh-BMP-7组在内侧部分的曼金评分在统计学上更低；在外侧部分，HYADD4-G与rh-BMP-2联合治疗组观察到显著更低的值。（B）HYADD4-G组的凯尔格伦和劳伦斯评分以及曼金评分均低于生理盐水组（P<0.002和P = 0.0031）。：这两项研究表明，HYADD4-G延缓了软骨退变，并且HYADD4-G与生长因子的联合具有协同作用。
