Paul Willi, Sharma Chandra P, Deb Kaushik Dilip
Division of Biosurface Technology, Biomedical Technology Wing Sree Chitra Tirunal Institute for Medical Sciences & Technology, Thiruvananthapuram 695012, India.
J Stem Cells. 2011;6(4):181-98.
Adult tissues contain quiescent reservoirs of multipotent somatic stem cells and pluripotent embryonic-like stem cells (ELSCs). Credited with regenerative properties gold is used across both -contemporary and -ancient medicines. Here, we show that gold exerted these effects by enhancing the pool of pluripotent ELSC while improving their stemness. We used hESCs as an in-vitro model to understand if gold could enhance self-renewal and pluripotency. Swarna-bhasma (SB), an ancient Indian gold microparticulate (41.1 nm), preparation, reduced spontaneous-differentiation, improved self-renewal, pluripotency and proliferation of hESCs. Colloidal gold-nanoparticles (GNP) (15.59 nm) were tested to confirm that the observations were attributable to nanoparticulate-gold. SB and GNP exposure: maintained -stemness, -karyotypic stability, enhanced pluripotency till day-12, increased average colony-sizes, and reduced the number of autonomously-derived differentiated FGFR1 positive fibroblast-niche-cells/colony. Particulate-gold induced upregulation of FGFR1 and IGF2 expression, and decrease in IGF1 secretion indicates IGF1/2 mediated support for enhanced pluripotency and self-renewal in hESCs.
成体组织含有多能性体干细胞和多能性胚胎样干细胞（ELSCs）的静止库。由于具有再生特性，黄金在现代医学和古代医学中都有应用。在这里，我们表明黄金通过增加多能性ELSC的数量并提高其干性来发挥这些作用。我们使用人胚胎干细胞（hESCs）作为体外模型来了解黄金是否能增强自我更新和多能性。印度古代的一种黄金微粒制剂Swarna-bhasma（SB，41.1纳米）减少了hESCs的自发分化，改善了其自我更新、多能性和增殖能力。对胶体金纳米颗粒（GNP，15.59纳米）进行了测试，以确认这些观察结果归因于纳米颗粒金。SB和GNP处理：维持了干性、核型稳定性，在第12天前增强了多能性，增加了平均集落大小，并减少了每个集落中自主分化的成纤维细胞龛细胞（FGFR1阳性）的数量。颗粒金诱导FGFR1和IGF2表达上调，IGF1分泌减少，表明IGF1/2介导支持hESCs增强多能性和自我更新。
