全基因组序列分析一株带有网状内皮组织增生病病毒 LTR 插入的重组马立克氏病病毒 GX0101 株。
Sequence analysis of the whole genome of a recombinant Marek's disease virus strain, GX0101, with a reticuloendotheliosis virus LTR insert.
机构信息
College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
出版信息
Arch Virol. 2013 Sep;158(9):2007-14. doi: 10.1007/s00705-013-1671-1. Epub 2013 Apr 4.
Marek's disease virus Chinese strain GX0101, isolated in 2001, is the first reported recombinant gallid herpesvirus type 2 (GaHV-2) field strain with one reticuloendotheliosis virus (REV) long terminal repeat (LTR) insert. We constructed an infectious bacterial artificial chromosome (BAC) clone of GX0101, which showed characteristics very similar to those of the parental virus in replication and pathogenicity. Using the GX0101 BAC clone, the complete genome of GX0101 was sequenced and analyzed. The length of the GX0101 genome is 178,101 bp, and it contains only one REV-LTR insert at a site 267 bp upstream of the sorf2 gene.
马立克氏病病毒中国株 GX0101 于 2001 年分离，是首例报道的重组禽疱疹病毒 2 型（GaHV-2）田间分离株，具有一个网状内皮组织增生病病毒（REV）长末端重复序列（LTR）插入。我们构建了 GX0101 的传染性细菌人工染色体（BAC）克隆，其在复制和致病性方面表现出与亲本病毒非常相似的特征。利用 GX0101 BAC 克隆，对 GX0101 的全基因组进行了测序和分析。GX0101 基因组的长度为 178101bp，仅在 sorf2 基因上游 267bp 处的一个位点有一个 REV-LTR 插入。
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