School of Oral and Dental Sciences, University of Bristol, Bristol, BS1 2LY, United Kingdom.
J Biomed Mater Res A. 2013 Nov;101(11):3247-55. doi: 10.1002/jbm.a.34622. Epub 2013 Apr 3.
The aim of this work is to investigate the use of microtopographies in providing physical cues to modulate the cellular response of human mesenchymal stem cells on ceramics. Two microgrooved patterns (100 μm/50 μm, 10 μm/10 μm groove/pitch) were transcribed reversely onto alumina green ceramic tapes via an embossing technique followed by sintering. Characterization of the micropatterned alumina surfaces and their cellular response was carried out. Spread and polygonal cell morphologies were observed on the wider groove (50 μm/100 μm) surface. Cells seeded onto the narrow groove (10 μm/10 μm) surface aligned themselves alongside the grooves, resulting in more elongated cell morphology. More osteoid matrix nodules shown by osteopontin and osteocalcin biomarkers were detected on the larger grooved surfaces after cell culture of 21 days, indicating a greater level of osteogenicity. This study has shown that micropatterned wider groove (50 μm) topographies are more suitable surfaces for improving osseointegration of ceramic implants.
本工作旨在研究微形貌在提供物理线索以调节人骨髓间充质干细胞在陶瓷上的细胞反应中的作用。通过压印技术将两种微沟图案（100μm/50μm、10μm/10μm 槽/间距）反转转录到氧化铝绿色陶瓷带上，然后进行烧结。对微图案化氧化铝表面及其细胞反应进行了表征。在较宽的沟槽（50μm/100μm）表面上观察到展开和多边形的细胞形态。在较窄的沟槽（10μm/10μm）表面上接种的细胞沿着沟槽排列，导致细胞形态更加细长。在细胞培养 21 天后，在较大沟槽表面上检测到更多由骨桥蛋白和骨钙素生物标志物显示的类骨质基质结节，表明成骨能力更高。这项研究表明，微图案化较宽沟槽（50μm）形貌更适合提高陶瓷植入物的骨整合。
