硫柳汞、甲基汞和氯化汞对Jurkat T细胞系的影响。

Effect of thimerosal, methylmercury, and mercuric chloride in Jurkat T Cell Line.

作者信息

Guzzi Gianpaolo, Pigatto Paolo D, Spadari Francesco, La Porta Caterina A M

机构信息

Italian Association for Metals and Biocompatibility Research - A.I.R.M.E.B., Via A. Banfi, 4, 20122 Milan, Italy.

出版信息

Interdiscip Toxicol. 2012 Sep;5(3):159-61. doi: 10.2478/v10102-012-0026-1.

DOI:10.2478/v10102-012-0026-1

PMID:23554557

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3600517/

Abstract

Mercury is a ubiquitous environmental toxicant that causes a wide range of adverse health effects in humans. Three forms of mercury exist: elemental, inorganic and organic. Each of them has its own profile of toxicity. The aim of the present study was to determine the effect of thimerosal, a topical antiseptic and preservative in vaccines routinely given to children, methyl mercury, and mercuric chloride on cellular viability measured by MTT in Jurkat T cells, a human T leukemia cell line. The treatment of Jurkat T cells with thimerosal caused a significant decrease in cellular viability at 1 μM (25%, p<0.05; IC50: 10 μM). Methyl mercury exhibited a significant decrease in cellular viability at 50 μM (33%, p<0.01; IC50: 65 μM). Mercuric chloride (HgCl2) did not show any significant change in cellular survival. Our findings showed that contrary to thimerosal and methyl mercury, mercuric chloride did not modify Jurkat T cell viability.

摘要

汞是一种普遍存在的环境毒物，会对人类健康造成广泛的不良影响。汞有三种形式：元素汞、无机汞和有机汞。它们各自具有不同的毒性特征。本研究的目的是确定硫柳汞（一种常用于儿童常规接种疫苗的局部防腐剂）、甲基汞和氯化汞对人T白血病细胞系Jurkat T细胞中通过MTT法测定的细胞活力的影响。用硫柳汞处理Jurkat T细胞后，在1μM时细胞活力显著下降（25%，p<0.05；IC50：10μM）。甲基汞在50μM时细胞活力显著下降（33%，p<0.01；IC50：65μM）。氯化汞（HgCl2）在细胞存活率方面未显示任何显著变化。我们的研究结果表明，与硫柳汞和甲基汞不同，氯化汞不会改变Jurkat T细胞的活力。