慢性特发性荨麻疹患者未受刺激的外周血单核细胞中细胞因子受体的mRNA谱

mRNA profiles of cytokine receptors in unstimulated peripheral blood mononuclear cells from patients with chronic idiopathic urticaria.

作者信息

Gao Jianming, Yang Aizhen, Chen Min, Li Ansheng, Yao Xu, Li Yumei, Xie Shihai, Yang Xueyuan, Zhong Liansheng, Chen Zhiqiang

机构信息

Department of Dermatology, Haidian Hospital, Beijing 10080, China ; Section of Clinical Immunology, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, Jiangsu, China.

出版信息

J Biomed Res. 2011 Mar;25(2):141-7. doi: 10.1016/S1674-8301(11)60018-3.

DOI:10.1016/S1674-8301(11)60018-3

PMID:23554682

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3596706/

Abstract

This present study was aimed to investigate the roles of the receptors of Th1/Th2 cytokines and chemokines in the pathogenesis of chronic idiopathic urticaria (CIU). Thirty patients with CIU, 30 patients with dermographism and 30 healthy controls were randomly enrolled. Reverse transcription-PCR (RT-PCR) was used to analyze the mRNA of cytokine receptors in peripheral blood mononuclear cells (PBMCs). The mRNA levels of tumor necrosis factor receptor (TNFR), interferon-γ receptor (IFN-γR), and interleukin-10 receptor (IL-10R) were statistically increased in the CIU group (P < 0.05), while IL-2R, IL-4R, IL-6R, and IL-13R showed no significant differences between the CIU and other groups. The mRNA levels of CCR3 and CCR6 were statistically increased in the CIU group (P < 0.05). The toll-like receptor 2 (TLR2) mRNA level was significantly lower in the CIU group than the healthy control group (P < 0.05). These findings indicate that the regulation of mRNA of TNFR, IFN-γR, IL-10R, CCR3, CCR6 and TLR2 may be involved in the pathogenesis of CIU.

摘要

本研究旨在探讨Th1/Th2细胞因子和趋化因子受体在慢性特发性荨麻疹（CIU）发病机制中的作用。随机纳入30例CIU患者、30例皮肤划痕症患者和30名健康对照者。采用逆转录聚合酶链反应（RT-PCR）分析外周血单个核细胞（PBMCs）中细胞因子受体的mRNA。CIU组肿瘤坏死因子受体（TNFR）、干扰素-γ受体（IFN-γR）和白细胞介素-10受体（IL-10R）的mRNA水平有统计学意义的升高（P<0.05），而IL-2R、IL-4R、IL-6R和IL-13R在CIU组与其他组之间无显著差异。CIU组CCR3和CCR6的mRNA水平有统计学意义的升高（P<0.05）。CIU组Toll样受体2（TLR2）的mRNA水平显著低于健康对照组（P<0.05）。这些发现表明，TNFR、IFN-γR、IL-10R、CCR3、CCR6和TLR2的mRNA调控可能参与了CIU的发病机制。