Gao Jianming, Yang Aizhen, Chen Min, Li Ansheng, Yao Xu, Li Yumei, Xie Shihai, Yang Xueyuan, Zhong Liansheng, Chen Zhiqiang
Department of Dermatology, Haidian Hospital, Beijing 10080, China ; Section of Clinical Immunology, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, Jiangsu, China.
J Biomed Res. 2011 Mar;25(2):141-7. doi: 10.1016/S1674-8301(11)60018-3.
This present study was aimed to investigate the roles of the receptors of Th1/Th2 cytokines and chemokines in the pathogenesis of chronic idiopathic urticaria (CIU). Thirty patients with CIU, 30 patients with dermographism and 30 healthy controls were randomly enrolled. Reverse transcription-PCR (RT-PCR) was used to analyze the mRNA of cytokine receptors in peripheral blood mononuclear cells (PBMCs). The mRNA levels of tumor necrosis factor receptor (TNFR), interferon-γ receptor (IFN-γR), and interleukin-10 receptor (IL-10R) were statistically increased in the CIU group (P < 0.05), while IL-2R, IL-4R, IL-6R, and IL-13R showed no significant differences between the CIU and other groups. The mRNA levels of CCR3 and CCR6 were statistically increased in the CIU group (P < 0.05). The toll-like receptor 2 (TLR2) mRNA level was significantly lower in the CIU group than the healthy control group (P < 0.05). These findings indicate that the regulation of mRNA of TNFR, IFN-γR, IL-10R, CCR3, CCR6 and TLR2 may be involved in the pathogenesis of CIU.
本研究旨在探讨Th1/Th2细胞因子和趋化因子受体在慢性特发性荨麻疹（CIU）发病机制中的作用。随机纳入30例CIU患者、30例皮肤划痕症患者和30名健康对照者。采用逆转录聚合酶链反应（RT-PCR）分析外周血单个核细胞（PBMCs）中细胞因子受体的mRNA。CIU组肿瘤坏死因子受体（TNFR）、干扰素-γ受体（IFN-γR）和白细胞介素-10受体（IL-10R）的mRNA水平有统计学意义的升高（P<0.05），而IL-2R、IL-4R、IL-6R和IL-13R在CIU组与其他组之间无显著差异。CIU组CCR3和CCR6的mRNA水平有统计学意义的升高（P<0.05）。CIU组Toll样受体2（TLR2）的mRNA水平显著低于健康对照组（P<0.05）。这些发现表明，TNFR、IFN-γR、IL-10R、CCR3、CCR6和TLR2的mRNA调控可能参与了CIU的发病机制。
