Alasandagutti Madhavi Latha, Ponnana Meenakshi, Sivangala Ramya, Thada Shruthi, Joshi Lavanya, Hussain Hidayath, Ansari Soheb Sadath, Valluri Vijayalakshmi, Gaddam Suman Latha
1 Immunology Department, Bhagwan Mahavir Medical Research Centre , Hyderabad, Andhra Pradesh, India .
Genet Test Mol Biomarkers. 2014 Dec;18(12):804-9. doi: 10.1089/gtmb.2014.0193.
Chronic Idiopathic Urticaria (CIU) is a common skin disorder, which may occur spontaneously. The aim of the present study was to assess the serum levels of interferon (IFN)-γ and interleukin (IL)-6 and to examine the association of IFN-γ+874 T/A and IL-6-174 G/C cytokine gene polymorphisms. To accomplish this, ELISA-based cytokine serum levels of IFN-γ (n=30) and IL-6 (n=30) in CIU patients (n=100) and Healthy Controls (HC) (n=200) were performed. Amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) was performed to verify the positional significance. A significant (p<0.0001) increase in the serum cytokine levels of IFN-γ and IL-6 was recorded in CIU patients compared to HC. The AT and TT genotypes of IFN-γ and GG genotype of IL-6 were found to be significantly associated with CIU. In conclusion, our findings show a significant increase in the cytokine levels of IFN-γ and IL-6, highlighting their regulatory role in the development of disease. In addition to this, association studies have revealed that TT genotype of IFN-γ +874 T/A and GG genotype of IL-6-174 G/C were susceptible towards the CIU.
慢性特发性荨麻疹（CIU）是一种常见的皮肤疾病，可能会自发出现。本研究的目的是评估血清干扰素（IFN）-γ和白细胞介素（IL）-6水平，并研究IFN-γ+874 T/A和IL-6-174 G/C细胞因子基因多态性的相关性。为实现这一目的，对100例CIU患者（n = 100）和200例健康对照者（HC）进行了基于酶联免疫吸附测定（ELISA）的IFN-γ（n = 30）和IL-6（n = 30）细胞因子血清水平检测。采用扩增阻滞突变系统-聚合酶链反应（ARMS-PCR）来验证其位置意义。与HC相比，CIU患者血清中IFN-γ和IL-6细胞因子水平显著升高（p<0.0001）。发现IFN-γ的AT和TT基因型以及IL-6的GG基因型与CIU显著相关。总之，我们的研究结果表明IFN-γ和IL-6的细胞因子水平显著升高，突出了它们在疾病发展中的调节作用。除此之外，关联研究显示IFN-γ +874 T/A的TT基因型和IL-6-174 G/C的GG基因型易患CIU。
