Coleman Allen Edward, McNeil Nicole, Kovalchuck Alexander Leonidovich, Wangsa Dara, Ried Thomas, Wang Hong
Department of Anesthesiology, Wayne State University, Detroit Medical Center, Detroit, Michigan 48201, USA;
J Biomed Res. 2012 Mar;26(2):117-24. doi: 10.1016/S1674-8301(12)60021-9.
Anesthesia is widely used in several medical settings and accepted as safe. However, there is some evidence that anesthetic agents can induce genomic changes leading to neural degeneration or apoptosis. Although chromosomal changes have not been observed in vivo, this is most likely due to DNA repair mechanisms, apoptosis, or cellular senescence. Potential chromosomal alterations after exposure to common anesthetic agents may be relevant in patients with genomic instability syndromes or with aggressive treatment of malignancies. In this study, the P388 murine B cells were cultured in vitro, and spectral karyotyping (SKY) was utilized to uncover genome-wide changes. Clinically relevant doses of cisatracurium and propofol increased structural and numerical chromosomal instability. These results may be relevant in patients with underlying chromosomal instability syndromes or concurrently being exposed to chemotherapeutic agents. Future studies may include utilization of stimulated peripheral blood lymphocytes to further confirm the significance of these results.
麻醉在多种医疗环境中被广泛使用且被认为是安全的。然而，有一些证据表明麻醉剂可诱导基因组变化，导致神经退行性变或细胞凋亡。尽管在体内尚未观察到染色体变化，但这很可能是由于DNA修复机制、细胞凋亡或细胞衰老所致。接触常见麻醉剂后潜在的染色体改变可能与患有基因组不稳定综合征的患者或接受恶性肿瘤积极治疗的患者有关。在本研究中，P388小鼠B细胞在体外培养，并利用光谱核型分析（SKY）来揭示全基因组变化。临床相关剂量的顺式阿曲库铵和丙泊酚增加了结构和数量染色体不稳定性。这些结果可能与患有潜在染色体不稳定综合征或同时接触化疗药物的患者有关。未来的研究可能包括利用刺激外周血淋巴细胞来进一步证实这些结果的重要性。
