Ishibashi Ken-ichi, Morita Mayu, Motoi Masuro, Liu Ying, Miura Noriko N, Adachi Yoshiyuki, Ohno Naohito
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo.
Int J Med Mushrooms. 2013;15(2):115-26. doi: 10.1615/intjmedmushr.v15.i2.10.
The aim of this work was to characterize an antibody response to β-glucan (BG), a major component of the fungal cell wall, at each isotype in human sera. The titer and reactivity of the anti-human BG antibody were examined using enzyme-linked immunosorbant assay plates coated with Candida soluble cell wall β-glucan as a standard antigen. The antibody was detected using anti-human immunoglobulin (Ig) G, IgM, and IgA. Its major class was IgG in all subjects. The antibody titer varied significantly. The anti-BG antibody showed greater reactivity to β-glucans derived from pathogenic fungi than monoglycosyl-branched β-glucans derived from mycelia culture medium. In addition, it was suggested that the anti-BG IgM antibody was bound relatively strongly to the β1,3-glucan backbone and the anti-BG IgG antibody to β1,6-glucan. The anti-BG antibody plays a variety of roles, according to class, in the host's response to fungi. We propose a new index of human response to β-glucan that effects the understanding of the response to β-glucan in humans.
这项工作的目的是在人血清中对真菌细胞壁的主要成分β-葡聚糖（BG）的每种同种型的抗体反应进行表征。使用包被念珠菌可溶性细胞壁β-葡聚糖作为标准抗原的酶联免疫吸附测定板检测抗人BG抗体的效价和反应性。使用抗人免疫球蛋白（Ig）G、IgM和IgA检测该抗体。在所有受试者中其主要类别为IgG。抗体效价差异显著。抗BG抗体对来自致病真菌的β-葡聚糖的反应性比对来自菌丝体培养基的单糖基分支β-葡聚糖的反应性更强。此外，提示抗BG IgM抗体与β1,3-葡聚糖主链结合相对较强，而抗BG IgG抗体与β1,6-葡聚糖结合。抗BG抗体根据类别在宿主对真菌的反应中发挥多种作用。我们提出了一种新的人类对β-葡聚糖反应的指标，这有助于理解人类对β-葡聚糖的反应。
