体外实验中胆汁盐的肝脏细胞毒性顺序与大鼠体内实验的结果不一致。

The order of hepatic cytotoxicity of bile salts in vitro does not agree with that examined in vivo in rats.

作者信息

Ohta M, Kanai S, Kitani K

机构信息

First Laboratory of Clinical Physiology, Tokyo Metropolitan Institute of Gerontology, Japan.

出版信息

Life Sci. 1990;46(21):1503-8. doi: 10.1016/0024-3205(90)90422-n.

DOI:10.1016/0024-3205(90)90422-n

PMID:2355795

Abstract

Using an enzyme release from isolated rat hepatocytes incubated with a bile salt as a marker, the cytotoxic order of bile salts was found to be taurochenodeoxycholate, glycochenodeoxycholate greater than tauroursodeoxycholate, glycoursodeoxycholate, cholate greater than taurocholate, glycocholate. Thus, the cytotoxicity of conjugates of ursodeoxycholate was greater than that of conjugates of cholate. However, these data do not agree with the order of cytotoxicity of these bile salts previously studied in vivo by the authors which demonstrated the least cytotoxic nature of conjugates of ursodeoxycholate.

摘要

以从与胆盐一起孵育的分离大鼠肝细胞中释放的酶作为标志物，发现胆盐的细胞毒性顺序为牛磺鹅去氧胆酸盐、甘氨鹅去氧胆酸盐大于牛磺熊去氧胆酸盐、甘氨熊去氧胆酸盐、胆酸盐大于牛磺胆酸盐、甘氨胆酸盐。因此，熊去氧胆酸盐缀合物的细胞毒性大于胆酸盐缀合物的细胞毒性。然而，这些数据与作者先前在体内研究的这些胆盐的细胞毒性顺序不一致，先前的研究表明熊去氧胆酸盐缀合物的细胞毒性最小。