Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
J Magn Reson. 2013 May;230:205-11. doi: 10.1016/j.jmr.2013.02.021. Epub 2013 Mar 15.
Robust and efficient band-selective magnetization transfer between CO and CA spins can be achieved in highly deuterated solid proteins by dipolar-based homonuclear cross polarization. The approach is designed for moderate magic-angle spinning rates and high external magnetic fields where the isotropic chemical shift difference of CO and CA considerably exceeds the spinning rate. The most efficient recoupling is achieved when the sum of effective radio-frequency fields on CO and CA resonances equals two times the spinning rate. This method can be directly implemented in proton-detected versions of inter-residual correlation experiments as needed for resonance assignment in protein solid-state NMR spectroscopy.
通过基于偶极相互作用的同核交叉极化，可以在高度氘代的固态蛋白质中实现 CO 和 CA 自旋之间稳健且高效的带选择性磁化转移。该方法适用于中等魔角旋转速率和高外磁场条件，此时 CO 和 CA 的各向同性化学位移差远远超过旋转速率。当 CO 和 CA 共振上的有效射频场之和等于旋转速率的两倍时，可实现最有效的再耦。这种方法可以直接在质子检测的残基间相关实验的版本中实现，以满足蛋白质固态 NMR 光谱学中共振分配的需要。
