Xiang ShengQi, Grohe Kristof, Rovó Petra, Vasa Suresh Kumar, Giller Karin, Becker Stefan, Linser Rasmus
Department for NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
J Biomol NMR. 2015 Jul;62(3):303-11. doi: 10.1007/s10858-015-9945-4. Epub 2015 May 15.
Proton detection in solid-state NMR has seen a tremendous increase in popularity in the last years. New experimental techniques allow to exploit protons as an additional source of information on structure, dynamics, and protein interactions with their surroundings. In addition, sensitivity is mostly improved and ambiguity in assignment experiments reduced. We show here that, in the solid state, sequential amide-to-amide correlations turn out to be an excellent, complementary way to exploit amide shifts for unambiguous backbone assignment. For a general assessment, we compare amide-to-amide experiments with the more common (13)C-shift-based methods. Exploiting efficient CP magnetization transfers rather than less efficient INEPT periods, our results suggest that the approach is very feasible for solid-state NMR.
在过去几年中，固态核磁共振中的质子检测受到了极大的关注。新的实验技术使得质子能够作为有关结构、动力学以及蛋白质与其周围环境相互作用的额外信息来源。此外，灵敏度大多得到了提高，并且在归属实验中的模糊性也降低了。我们在此表明，在固态中，酰胺到酰胺的顺序相关性结果证明是利用酰胺化学位移进行明确的主链归属的一种出色的补充方法。为了进行总体评估，我们将酰胺到酰胺实验与更常见的基于（13）C化学位移的方法进行了比较。利用高效的交叉极化（CP）磁化转移而非效率较低的INEPT序列，我们的结果表明该方法对于固态核磁共振非常可行。
