Sakarya University, Faculty of Science and Letters, Department of Biology, Sakarya-Turkey.
Biol Res. 2012;45(4):331-6. doi: 10.4067/S0716-97602012000400001.
In sexually reproducing organisms, primordial germ cells (PGCs) give rise to the cells of the germ line, the gametes. In many animals, PGCs are set apart from somatic cells early during embryogenesis. This study explores the origin of primordial germ cells (PGCs) of the zebra fish and examines their morphology during early development (1st day-15th day). PGCs were selectively stained by the alkaline phosphatase histochemical reaction and viewed by light and electron microscopy from the time they are first detectable in the yolk sac endoderm. PGCs occurred in the subendodermal space on the syncytial periblast; differing from the surrounding endodermal cells. Later the PGCs moved to between the blastoderm and yolk sac and transferred to the dorsal mesentery where they formed gonadal anlage with mesoderm cells. PGCs were easily distinguished from somatic cells by their morphology and low electron density of their nuclei. Under light microscopy, PCGs were rounded with a distinct cytoplasmic membrane.
在有性生殖的生物中，原始生殖细胞（PGCs）产生生殖细胞系的细胞，即配子。在许多动物中，PGCs 在胚胎发生早期就与体细胞区分开来。本研究探讨了斑马鱼原始生殖细胞（PGCs）的起源，并检查了它们在早期发育（第 1 天至第 15 天）期间的形态。通过碱性磷酸酶组织化学反应选择性地对 PGCs 进行染色，并从它们在卵黄囊内胚层中首次可检测到时开始通过光镜和电子显微镜进行观察。PGCs 出现在合胞体周质的下内皮空间中；与周围的内胚层细胞不同。后来，PGCs 移动到胚层和卵黄囊之间，并转移到背系膜，在那里它们与中胚层细胞一起形成性腺原基。PGCs 可以通过其形态和核的低电子密度与体细胞轻易区分开来。在光镜下，PCGs 呈圆形，具有明显的细胞质膜。
