Barrell Philippa, Grossniklaus Ueli
New Zealand Institute for Plant and Food Research, Christchurch, New Zealand.
Methods Mol Biol. 2013;990:45-52. doi: 10.1007/978-1-62703-333-6_5.
Meiosis is a key step in sexual plant reproduction. Cytological analysis of meiosis enables hypotheses of gene function during meiosis to be confirmed or discarded. Observation of meiosis in the female reproductive organs in many higher plants is technically challenging because the cells are enclosed deep within the nucellus and ovule tissues. In this chapter, we describe a simple staining procedure using Schiff's reagent optimized for use with confocal microscopy to observe meiosis within intact ovules of Zea mays (maize). Using this procedure we present images taken by confocal laser scanning microscopy of wild-type meiotic cells in whole-mount maize ovules.
减数分裂是有性植物繁殖中的关键步骤。对减数分裂进行细胞学分析能够证实或否定关于减数分裂过程中基因功能的假说。在许多高等植物的雌性生殖器官中观察减数分裂在技术上具有挑战性，因为细胞被包裹在珠心和胚珠组织的深处。在本章中，我们描述了一种使用席夫试剂的简单染色程序，该程序经过优化可用于共聚焦显微镜，以观察玉米完整胚珠内的减数分裂。利用这个程序，我们展示了通过共聚焦激光扫描显微镜拍摄的玉米整胚珠中野生型减数分裂细胞的图像。
