Fonterra Co-operative Group Ltd., P.O. Box 7, Waitoa 3380, New Zealand.
Anal Bioanal Chem. 2013 Jun;405(15):5311-9. doi: 10.1007/s00216-013-6935-9. Epub 2013 Apr 5.
A method for the simultaneous analysis of nucleosides and nucleotides in infant formula using reversed-phase liquid chromatography-tandem mass spectrometry is described. This approach is advantageous for compliance testing of infant formula over other LC-MS methods in which only nucleotides or nucleosides are measured. Following sample dissolution, protein was removed by centrifugal ultrafiltration. Chromatographic analyses were performed using a C18 stationary phase and gradient elution of an ammonium acetate/bicarbonate buffer, mass spectrometric detection and quantitation by a stable isotope-labelled internal standard technique. A single laboratory validation was performed, with spike recoveries of 80.1-112.9% and repeatability relative standard deviations of 1.9-7.2%. Accuracy as bias was demonstrated against reference values for NIST1849a certified reference material. The method has been validated for the analysis of bovine milk-based, soy-based, caprine milk-based and hydrolysed milk protein-based infant formulae.
本文描述了一种使用反相液相色谱-串联质谱法同时分析婴儿配方食品中核苷和核苷酸的方法。与其他仅测量核苷酸或核苷的 LC-MS 方法相比，这种方法有利于婴儿配方食品的合规性测试。在样品溶解后，通过离心超滤去除蛋白质。使用 C18 固定相和乙酸铵/碳酸氢盐缓冲液的梯度洗脱进行色谱分析，通过稳定同位素标记内标技术进行质谱检测和定量。进行了单实验室验证，加标回收率为 80.1-112.9%，重复性相对标准偏差为 1.9-7.2%。针对 NIST1849a 认证参考物质的参考值，证明了方法的准确性。该方法已针对基于牛乳、大豆、山羊乳和水解乳蛋白的婴儿配方食品进行了验证。
