唾液 microRNAs 作为食管癌检测有前途的生物标志物。
Salivary microRNAs as promising biomarkers for detection of esophageal cancer.
机构信息
Department of Gastroenterology, Guangdong Academy of Medical Sciences, Guangdong General Hospital, Guangzhou City, Guangdong Province, China.
出版信息
PLoS One. 2013;8(4):e57502. doi: 10.1371/journal.pone.0057502. Epub 2013 Apr 1.
BACKGROUND AND PURPOSE
Tissue microRNAs (miRNAs) can detect cancers and predict prognosis. Several recent studies reported that tissue, plasma, and saliva miRNAs share similar expression profiles. In this study, we investigated the discriminatory power of salivary miRNAs (including whole saliva and saliva supernatant) for detection of esophageal cancer.
MATERIALS AND METHODS
By Agilent microarray, six deregulated miRNAs from whole saliva samples from seven patients with esophageal cancer and three healthy controls were selected. The six selected miRNAs were subjected to validation of their expression levels by RT-qPCR using both whole saliva and saliva supernatant samples from an independent set of 39 patients with esophageal cancer and 19 healthy controls.
RESULTS
Six miRNAs (miR-10b*, miR-144, miR-21, miR-451, miR-486-5p, and miR-634) were identified as targets by Agilent microarray. After validation by RT-qPCR, miR-10b*, miR-144, and miR-451 in whole saliva and miR-10b*, miR-144, miR-21, and miR-451 in saliva supernatant were significantly upregulated in patients, with sensitivities of 89.7, 92.3, 84.6, 79.5, 43.6, 89.7, and 51.3% and specificities of 57.9, 47.4, 57.9%, 57.9, 89.5, 47.4, and 84.2%, respectively.
CONCLUSIONS
We found distinctive miRNAs for esophageal cancer in both whole saliva and saliva supernatant. These miRNAs possess discriminatory power for detection of esophageal cancer. Because saliva collection is noninvasive and convenient, salivary miRNAs show great promise as biomarkers for detection of esophageal cancer in areas at high risk.
背景与目的
组织 microRNAs（miRNAs）可用于检测癌症并预测预后。最近的几项研究报告称，组织、血浆和唾液中的 miRNAs 具有相似的表达谱。在这项研究中，我们研究了唾液 miRNAs（包括全唾液和唾液上清液）在检测食管癌中的区分能力。
材料与方法
通过 Agilent 微阵列，从 7 名食管癌患者和 3 名健康对照者的全唾液样本中选择了 6 个下调的 miRNAs。使用来自 39 名食管癌患者和 19 名健康对照者的全唾液和唾液上清液样本的 RT-qPCR 验证了这 6 个选定 miRNAs 的表达水平。
结果
Agilent 微阵列鉴定出 6 个 miRNAs（miR-10b*、miR-144、miR-21、miR-451、miR-486-5p 和 miR-634）为靶标。通过 RT-qPCR 验证后，全唾液中的 miR-10b*、miR-144 和 miR-451，以及唾液上清液中的 miR-10b*、miR-144、miR-21 和 miR-451 在患者中显著上调，其敏感性分别为 89.7%、92.3%、84.6%、79.5%、43.6%、89.7%和 51.3%，特异性分别为 57.9%、47.4%、57.9%、57.9%、89.5%、47.4%和 84.2%。
结论
我们在全唾液和唾液上清液中都发现了食管癌特异的 miRNAs。这些 miRNAs 具有检测食管癌的区分能力。由于唾液采集是非侵入性和方便的，唾液 miRNAs 作为高危地区食管癌检测的生物标志物具有很大的应用前景。
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