Medical Genetics, Meram Medical Faculty, Konya University, Konya, Turkey.
Int J Immunogenet. 2013 Dec;40(6):482-7. doi: 10.1111/iji.12056. Epub 2013 Apr 7.
This study was designed to determine the possible asssociation between selected FAS and FASLG polymorphisms and Hepatitis B virus (HBV) infection. FAS-670 G/A, FAS-1377 G/A, FASLG-844 T/C and FASLG IVS2nt-124 A/G polymorphisms were genotyped by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). A total of age and sex matched 108 controls and a hundred chronic HBV patients were recruited to conduct a case-control study. FAS-670 polymorphism was associated with chronic HBV infection (P = 0.03) FAS-1377 GG, GA and AA genotypes among the cases (90%, 5% and 5%, respectively) were significantly different from those among the controls (68%, 31.5% and 5.6%; P = 0.00). FASLG-844 allele distribution was similar between the groups (P = 0.17) but TC genotype (67.3%) was frequent in chronic HBV patients, while CC genotype was found significantly higher (29.6%) in controls. No association between FASLG IVS2nt-124 polymorphism and chronic HBV infection could be identified (P = 0.55). FAS-670 polymorphism is associated with chronic HBV infection, while FASLG IVS2nt-124 A/G polymorphism is not. The FAS-1377G/A and FASLG-844 T/C genotypes are likely to play a substantial role in HBV infection. Further studies evaluating polymorphisms in other genes related with apoptosis are needed to elucidate the role of genetic variation in HBV infection.
本研究旨在确定 FAS 和 FASLG 多态性与乙型肝炎病毒（HBV）感染之间的可能关联。通过聚合酶链反应和限制性片段长度多态性（PCR-RFLP）对 FAS-670 G/A、FAS-1377 G/A、FASLG-844 T/C 和 FASLG IVS2nt-124 A/G 多态性进行基因分型。共招募了年龄和性别匹配的 108 名对照和 100 名慢性乙型肝炎患者进行病例对照研究。FAS-670 多态性与慢性 HBV 感染相关（P=0.03），病例组的 FAS-1377 GG、GA 和 AA 基因型（分别为 90%、5%和 5%）与对照组的基因型（68%、31.5%和 5.6%；P=0.00）明显不同。两组间 FASLG-844 等位基因分布相似（P=0.17），但 TC 基因型（67.3%）在慢性 HBV 患者中较为常见，而 CC 基因型在对照组中明显较高（29.6%）。FASLG IVS2nt-124 多态性与慢性 HBV 感染无相关性（P=0.55）。FAS-670 多态性与慢性 HBV 感染相关，而 FASLG IVS2nt-124 A/G 多态性则无相关性。FAS-1377G/A 和 FASLG-844 T/C 基因型可能在 HBV 感染中起重要作用。需要进一步研究评估与凋亡相关的其他基因中的多态性，以阐明遗传变异在 HBV 感染中的作用。
