Jiménez Félix, Barbaglia Yanina, Bucci Pamela, Tedeschi Fabián A, Zalazar Fabián E
Servicio de Gastroenterología, Hospital Dr. J. M. Cullen, Santa Fe, Argentina.
Rev Argent Microbiol. 2013 Jan-Mar;45(1):39-43.
Our goals were: a) to detect Helicobacter pylori in gastric biopsies of symptomatic adults by PCR, b) to detect the presence of the cagA gene as well as of the allelic variants of the vacA gene, and c) to correlate genotypes with the endoscopic diagnoses. H. pylori was detected in 81 % (39/48) of patients by nested PCR for hsp60. The presence of cagA was detected in 15/22 of samples and vacA s1 - m1 was the most frequent allelic combination (15/22). Gastritis, the most frequent diagnosis, was associated with genotype cagA+ in 10/13 of patients. In this group, 9/13 showed the allelic variant vacA s1- m1. The variant vacA s2 - m2 was detected in 3/3 of gastritis cases by H. pylori with the cagA- genotype. These results are the first reported in our region and provide data of epidemiological interest.
a) 通过聚合酶链反应（PCR）检测有症状成年人胃活检组织中的幽门螺杆菌；b) 检测细胞毒素相关基因A（cagA）以及空泡毒素A（vacA）基因的等位基因变体的存在；c) 将基因型与内镜诊断结果相关联。通过针对热休克蛋白60（hsp60）的巢式PCR，在81%（39/48）的患者中检测到幽门螺杆菌。在15/22的样本中检测到cagA的存在，且vacA s1 - m1是最常见的等位基因组合（15/22）。胃炎是最常见的诊断结果，在10/13的患者中与cagA+基因型相关。在该组中，9/13显示为等位基因变体vacA s1 - m1。在3/3例由cagA - 基因型幽门螺杆菌引起的胃炎病例中检测到vacA s2 - m2变体。这些结果是我们地区首次报道的，提供了具有流行病学意义的数据。
