大鼠肠道中的一种胆囊收缩素代谢酶。

A cholecystokinin-metabolizing enzyme in rat intestine.

作者信息

Turkelson C M, Solomon T E, Hamilton J

机构信息

Veterans Administration Medical Center, Kansas City, MO 64128.

出版信息

Peptides. 1990 Mar-Apr;11(2):213-9. doi: 10.1016/0196-9781(90)90073-e.

DOI:10.1016/0196-9781(90)90073-e

PMID:2356152

Abstract

Acid extracts of rat intestine contain a material which metabolizes cholecystokinin-33 (CCK-33) to CCK-12. Soybean trypsin inhibitor had little effect on CCK metabolism by the intestinal material. The molecular weight of the CCK-metabolizing activity, estimated by gel filtration, was 34,000. These data suggest rat intestine contains a nontrypsin CCK-metabolizing enzyme. Results from gel filtration also suggest that large CCK forms can be artifactually degraded to smaller ones during chromatography.

摘要

大鼠肠道的酸性提取物含有一种能将胆囊收缩素-33（CCK-33）代谢为CCK-12的物质。大豆胰蛋白酶抑制剂对肠道物质的CCK代谢影响很小。通过凝胶过滤估计，CCK代谢活性的分子量为34000。这些数据表明大鼠肠道含有一种非胰蛋白酶的CCK代谢酶。凝胶过滤的结果还表明，在色谱分析过程中，较大的CCK形式可能会人为地降解为较小的形式。