斯托克斯拉曼散射在时间相关单光子计数中的应用：酪氨酸盐荧光寿命的应用。
The use of Stokes Raman scattering in time correlated single photon counting: application to the fluorescence lifetime of tyrosinate.
作者信息
Willis K J, Szabo A G, Krajcarski D T
机构信息
Division of Biological Sciences, National Research Council of Canada, Ottawa, Ontario.
出版信息
Photochem Photobiol. 1990 Mar;51(3):375-7. doi: 10.1111/j.1751-1097.1990.tb01725.x.
Abstract
The decay kinetics of the tyrosinate form of tyrosine in aqueous solution, pH 11, were investigated by time correlated single photon counting using laser excitation. The lifetime of the single exponential decay was 30.5 +/- 0.2 ps. Stokes Raman scattering was used to determine the instrument response function and the advantages of this approach over the conventional Rayleigh scattering method are discussed.
摘要
在pH值为11的水溶液中，通过激光激发的时间相关单光子计数法研究了酪氨酸酪氨酸盐形式的衰变动力学。单指数衰变的寿命为30.5±0.2皮秒。利用斯托克斯拉曼散射来确定仪器响应函数，并讨论了这种方法相对于传统瑞利散射方法的优势。
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