Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing, China.
Int J Gynaecol Obstet. 2013 Jul;122(1):5-8. doi: 10.1016/j.ijgo.2013.02.015. Epub 2013 Apr 4.
To identify possible biomarkers for the clinical grading of intrahepatic cholestasis of pregnancy (ICP) through serum bile acid (SBA) profiling in women with ICP.
Serum samples were collected in the last trimester of pregnancy from 33 women with severe ICP, 28 women with mild ICP, and 35 women with a normal pregnancy. The SBA levels were determined by high-performance liquid chromatography-tandem mass spectrometry.
Patients with severe ICP had significantly higher serum levels of taurochenodeoxycholic acid, tauroursodeoxycholic acid, glycocholic acid (GCA), taurocholic acid (TCA), and glycochenodeoxycholic acid than women with mild ICP or a normal pregnancy. Primary bile acid species, in particular TCA and GCA, were the main bile acids detected and their levels were significantly higher in the severe ICP group than in the other 2 groups.
There is an obvious difference in the SBA profiles of women with severe ICP and those with mild ICP, indicating that primary bile acids may be useful biomarkers for the clinical grading of ICP. Implementation of primary bile acid testing in clinical practice may help clinicians to determine the appropriate management strategy for patients with ICP.
通过对妊娠期肝内胆汁淤积症（ICP）患者的血清胆汁酸（SBA）谱分析，确定可能的生物标志物，用于 ICP 的临床分级。
在妊娠晚期，收集了 33 例重度 ICP 患者、28 例轻度 ICP 患者和 35 例正常妊娠患者的血清样本。采用高效液相色谱-串联质谱法测定 SBA 水平。
重度 ICP 患者的牛磺胆酸、牛磺熊脱氧胆酸、甘氨胆酸（GCA）、胆酸（TCA）和甘氨鹅脱氧胆酸血清水平明显高于轻度 ICP 患者或正常妊娠患者。初级胆汁酸种类，特别是 TCA 和 GCA，是主要检测到的胆汁酸，其水平在重度 ICP 组明显高于其他 2 组。
重度 ICP 患者和轻度 ICP 患者的 SBA 谱明显不同，提示初级胆汁酸可能是 ICP 临床分级的有用生物标志物。在临床实践中实施初级胆汁酸检测可能有助于临床医生为 ICP 患者确定适当的管理策略。
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