INRA, UMR 1347 Agroécologie, Pôle GEAPSI, BP 86510, 21065 Dijon Cedex, France.
Biotechnol Adv. 2013 Dec;31(8):1237-46. doi: 10.1016/j.biotechadv.2013.03.008. Epub 2013 Apr 3.
The use and exploitation of electrophysiology with plant cells have witnessed a slow but steady increase for a number of purposes in recent years. First envisaged only as a tool for the recovery of somatic hybrid plants following protoplast electrofusion, or for transient and/or stable genetic transformation following electroporation-mediated entry of foreign genes into protoplasts and cells, electrophysiological studies with plant cells and tissues have since spanned into other areas, and particularly for the assessment of the possible effects of electric and electromagnetic fields on the subsequent growth and differentiation competences of the electro-treated cells. This review will critically discuss these various applications of electrophysiology and will also aim at analysing the fundamental physiological and physico-chemical mechanisms underlying them.
近年来，植物细胞的电生理学的应用和开发已经在许多方面取得了缓慢但稳定的进展。最初，它仅被视为原生质体电融合后体细胞杂种植物恢复的一种手段，或者是电穿孔介导的外源基因进入原生质体和细胞后瞬时和/或稳定遗传转化的一种手段。然而，此后，植物细胞和组织的电生理学研究已经扩展到其他领域，特别是用于评估电场和电磁场对电处理细胞后续生长和分化能力的可能影响。本文将批判性地讨论电生理学的这些应用，并分析其背后的基本生理和物理化学机制。
