委内瑞拉类圆线虫实验性感染中的寄生虫学和分子诊断
Parasitological and molecular diagnosis in experimental Strongyloides venezuelensis infection.
作者信息
Paula Fabiana Martins, Sitta Renata Barnabé, Malta Fernanda Mello, Gottardi Maiara, Corral Marcelo Andreetta, Gryschek Ronaldo César Borges, Chieffi Pedro Paulo
机构信息
Hospital das Clínicas da Faculdade de Medicina, Instituto de Medicina Tropical de São Paulo, USP, São Paulo, SP, Brazil.
出版信息
Rev Inst Med Trop Sao Paulo. 2013 Mar-Apr;55(2):141-3. doi: 10.1590/s0036-46652013000200015.
Strongyloides venezuelensis is a parasitic nematode of rats which is frequently used as a model to study human and animal strongyloidiasis. The aim of this study was to evaluate the correlation between parasitological and molecular diagnosis in Strongyloides venezuelensis infection. PCR assays were used to detect S. venezuelensis DNA in fecal samples obtained from experimentally infected Rattus norvegicus. The results showed a higher sensitivity of the PCR assay in detecting the infection compared to parasitological methods.
委内瑞拉类圆线虫是大鼠的一种寄生线虫，常被用作研究人类和动物类圆线虫病的模型。本研究的目的是评估委内瑞拉类圆线虫感染中寄生虫学诊断与分子诊断之间的相关性。采用聚合酶链反应（PCR）检测法，对实验感染褐家鼠粪便样本中的委内瑞拉类圆线虫DNA进行检测。结果显示，与寄生虫学方法相比，PCR检测法在检测感染方面具有更高的灵敏度。
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