Silva Laryssa Pinheiro Costa, Ferraz Carolina Magri, Aguiar Anderson Rocha, Araújo Jackson Victor, Ribeiro Steveen Rios, Rossi Débora Goldner, Mendes Luanderson Queiroz, Pereira Fausto Edmundo Lima, Moreira Narcisa Imaculada Brant, Braga Fabio Ribeiro
Department of Parasitology, University of Vila Velha-ES, Vila Velha, ES, Brazil.
Department of Veterinary Medicine, Federal University of Viçosa, Viçosa, MG, Brazil.
Parasitol Res. 2017 Jul;116(7):2047-2051. doi: 10.1007/s00436-017-5502-2. Epub 2017 May 15.
Strongyloidiasis is the most clinically important disease among the infections caused by geohelminths, seeing that this parasite can cause autoinfection. The use of nematophagous fungi like Duddingtonia flagrans, that have predation action on eggs and infecciososas forms of helminths, emerges as an alternative method for environmental control. For this reason, analyzing the viability of larvae and eggs of Strongyloides venezuelensis and the action of Duddingtonia flagrans AC001 in vermiculite, as well as the action of the nematophagous fungi in different growth stages, is important to elaborate and define the best culture conditions that favor the activity of the fungus. Two different growth conditions were applied: both eggs and AC001 fungi were added at the same time to the vermiculite (assay A) and the addition of eggs after the growth of the AC001 fungi in the vermiculite (assay B). To recover the L larvae, the Baermann-Moraes method was applied, followed by the counting of L dead and alive. At last, it was observed that the vermiculite enriched with organic material is an adequate culture medium not only for the growth of the S. venezuelensis but also for the growth of the D. flagrans fungus, being therefore, a satisfactory culture medium for tests of viability and predatory action of this fungus. It was also observed that the activity of the AC001 fungus is greater when it is growing concomitantly with the eggs, in other words, when it is in the adaptation phase.
粪类圆线虫病是土源性蠕虫感染中临床上最重要的疾病，因为这种寄生虫可导致自身感染。使用像少孢节丛孢菌这样的食线虫真菌，其对蠕虫的虫卵和感染性形态具有捕食作用，成为一种环境控制的替代方法。因此，分析委内瑞拉粪类圆线虫幼虫和虫卵的活力以及少孢节丛孢菌AC001在蛭石中的作用，以及食线虫真菌在不同生长阶段的作用，对于精心制定和确定有利于真菌活性的最佳培养条件很重要。应用了两种不同的生长条件：将虫卵和AC001真菌同时添加到蛭石中（试验A），以及在AC001真菌在蛭石中生长后添加虫卵（试验B）。为了回收L期幼虫，采用了贝尔曼-莫赖斯方法，随后对死亡和存活的L期幼虫进行计数。最后观察到，富含有机物质的蛭石不仅是委内瑞拉粪类圆线虫生长的合适培养基，也是少孢节丛孢菌生长的合适培养基，因此，是用于该真菌活力和捕食作用测试的令人满意的培养基。还观察到，AC001真菌在与虫卵同时生长时，即在适应阶段，其活性更大。
