Gorgani Tahmine, Naem Soraya, Farshid Amir Abbass, Otranto Domenico
Parasit Vectors. 2013 Apr 8;6:87. doi: 10.1186/1756-3305-6-87.
Physaloptera clausa (Spirurida: Physalopteridae) nematodes parasitize the stomach of the European hedgehog (Erinaceus europaeus) and cause weight loss, anorexia and gastric lesions. The present study provides the first morphological description of adult P. clausa from the stomachs of infected hedgehogs, using scanning electron microscopy (SEM).
From June to October 2011, 10 P. clausa from European hedgehogs were fixed, dried, coated and subjected to SEM examination.
Males and females (22-30 mm and 28-47 mm, respectively) were stout, with the cuticle reflecting over the lips to form a large cephalic collarette and showing fine transverse striations in both sexes. The mouth was characterized by two large, simple triangular lateral pseudolabia, each armed with external and internal teeth. Inside the buccal cavity, a circle of internal small teeth can be observed. Around the mouth, four sub-median cephalic papillae and two large amphids were also observed. The anterior end of both male and female bore an excretory pore on the ventral side and a pair of lateral ciliated cervical papillae. In the female worm, the vulva was located in the middle and the eggs were characterized by smooth surfaces. The posterior end of the female worm was stumpy with two large phasmids in proximity to its extremity. The posterior end of the male had large lateral alae, joined together anteriorly across the ventral surface, with subequal and dissimilar spicules, as well as four pairs of stalked pre-cloacal papillae, three pairs of post-cloacal papillae, and two phasmids. Three sessile papillae occured anteriorly and four posteriorly to the cloaca.
The present SEM study provides the first in-depth morphological characterization of adult P. clausa, and highlights similarities and differences with P. bispiculata P. herthameyerae, Heliconema longissimum and Turgida turgida.
克劳氏泡翼线虫（旋尾目：泡翼科）寄生于欧洲刺猬（ Erinaceus europaeus ）的胃内，可导致体重减轻、厌食和胃部病变。本研究首次利用扫描电子显微镜（SEM）对感染刺猬胃内的成年克劳氏泡翼线虫进行了形态学描述。
2011年6月至10月，对10条来自欧洲刺猬的克劳氏泡翼线虫进行固定、干燥、镀膜并进行扫描电子显微镜检查。
雄虫和雌虫（分别为22 - 30毫米和28 - 47毫米）身体粗壮，角质层在唇部上方反折形成一个大的头部项圈，两性均有细微的横向条纹。口部的特征是有两个大的、简单的三角形侧假唇，每个假唇都有外齿和内齿。在口腔内部，可以观察到一圈内部小齿。在口周围，还观察到四个亚中位头部乳突和两个大的化感器。雄虫和雌虫的前端腹侧都有一个排泄孔和一对侧生纤毛颈乳突。在雌虫中，阴门位于中部，虫卵表面光滑。雌虫后端短粗，在其末端附近有两个大的尾感器。雄虫后端有大的侧叶膜，在腹面联合于前端，有不等长且不同的交合刺，以及四对有柄的泄殖腔前乳突、三对泄殖腔后乳突和两个尾感器。泄殖腔前方有三个无柄乳突，后方有四个。
本扫描电子显微镜研究首次对成年克劳氏泡翼线虫进行了深入的形态学特征描述，并突出了其与双刺泡翼线虫、赫氏泡翼线虫、长形螺旋线虫和肿胀螺旋线虫的异同。
