Cancer Immunology Research Unit, The Canberra Hospital, Woden, Australian Capital Territory, Australia.
J Immunol Methods. 2013 Jun 28;392(1-2):68-70. doi: 10.1016/j.jim.2013.03.010. Epub 2013 Apr 6.
In this technical note we provide data useful for the clinical application of the target-induced Natural Killer (NK) loss (TINKL) assay. The TINKL assay is a sensitive flow cytometry-based assay for measuring NK cell function. Loss of NK cells from the lymphocyte gate occurs following culture with K562 (the prototypic target cell for natural killing) and antibody-coated target cells (for antibody-dependent killing). By analysis of multiple samples of PBMC from single donors we document the intra-experimental variability and the inter-experimental variability of the assay. The intra-experimental coefficient of variation (CV) was on average 11% for natural killing and 3% for antibody-dependent killing, compared to 14% and 9% respectively for the inter-experimental variation. Analysis of a 123 normal healthy adults shows large variability in the functional capacity of NK cells in the population both for natural killing (CV 33%) and antibody-dependent killing (CV 27%).
在本技术说明中，我们提供了与靶诱导自然杀伤 (NK) 细胞耗竭 (TINKL) 测定的临床应用相关的数据。TINKL 测定是一种基于流式细胞术的灵敏方法，用于测量 NK 细胞功能。在用 K562（天然杀伤的典型靶细胞）和抗体包被的靶细胞（用于抗体依赖性杀伤）孵育后，从淋巴细胞门中损失 NK 细胞。通过对来自单个供体的多个 PBMC 样本进行分析，我们记录了该测定的实验内变异性和实验间变异性。实验内的变异系数 (CV) 平均为自然杀伤的 11%和抗体依赖性杀伤的 3%，而实验间变异性分别为 14%和 9%。对 123 名健康成年人的分析表明，NK 细胞的功能能力在人群中存在很大的变异性，无论是自然杀伤 (CV 33%) 还是抗体依赖性杀伤 (CV 27%)。
