Src同源2结构域对磺基酪氨酸和磷酸酪氨酸的分子识别

Molecular recognition of sulfotyrosine and phosphotyrosine by the Src homology 2 domain.

作者信息

Ju Tong, Niu Wei, Cerny Ronald, Bollman Joshua, Roy Anthony, Guo Jiantao

机构信息

Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.

出版信息

Mol Biosyst. 2013 Jul;9(7):1829-32. doi: 10.1039/c3mb70061e. Epub 2013 Apr 9.

DOI:10.1039/c3mb70061e

PMID:23567872

Abstract

We study molecular recognition of two closely resembling posttranslational modification products, phosphotyrosine (pTyr) and sulfotyrosine (sTyr). The differences in charge, size, and binding geometry of pTyr and sTyr are the main recognition elements. Protein engineering can be applied to alter the specificity and strength of the recognition.

摘要

我们研究了两种极为相似的翻译后修饰产物——磷酸酪氨酸（pTyr）和磺基酪氨酸（sTyr）的分子识别。pTyr和sTyr在电荷、大小和结合几何结构上的差异是主要的识别元件。蛋白质工程可用于改变识别的特异性和强度。