Lin Ying-Li, Ma Jian-Hua, Luo Xiang-Li, Guan Tong-Yu, Li Zhi-Gang
Department of Urology, Xuzhou Tumour Hospital (Xuzhou Third People's Hospital), Xuzhou, Jiangsu Province, China.
J Int Med Res. 2013 Feb;41(1):48-54. doi: 10.1177/0300060513475571. Epub 2013 Jan 23.
To investigate the clinical significance of protocadherin-8 (PCDH8) promoter methylation in bladder cancer.
Methylation-specific polymerase chain reaction was used to examine the promoter methylation status of PCDH8 in tumour tissue samples obtained from patients with bladder cancer, and in normal bladder epithelial tissue samples obtained from age- and sex-matched control subjects. Methylation status was correlated with demographic, clinical and pathological parameters and disease outcome.
PCDH8 promoter methylation was detected in 76/135 (56.3%) patients with bladder cancer and none of 34 (0%) control subjects. Methylation was significantly associated with advanced stage (T2-T4), high grade (G3), tumour recurrence, larger tumour diameter (>3 cm) and nonpapillary morphology. In addition, methylation was associated with significantly shorter survival time and was an independent predictor of overall survival.
PCDH8 promoter methylation is a common occurrence in bladder cancer, and is associated with malignant behaviour and poor prognosis. Determination of PCDH8 promoter methylation status in tumour tissue may assist in the identification of patients who require aggressive postoperative intervention in order to improve prognosis.
探讨原钙黏蛋白8（PCDH8）启动子甲基化在膀胱癌中的临床意义。
采用甲基化特异性聚合酶链反应检测膀胱癌患者肿瘤组织样本以及年龄和性别匹配的对照受试者正常膀胱上皮组织样本中PCDH8的启动子甲基化状态。甲基化状态与人口统计学、临床和病理参数以及疾病转归相关。
在135例膀胱癌患者中的76例（56.3%）检测到PCDH8启动子甲基化，而34例对照受试者（0%）均未检测到。甲基化与晚期（T2 - T4）、高级别（G3）、肿瘤复发、肿瘤直径较大（>3 cm）和非乳头状形态显著相关。此外，甲基化与生存时间显著缩短相关，并且是总生存的独立预测因素。
PCDH8启动子甲基化在膀胱癌中常见，且与恶性行为和不良预后相关。检测肿瘤组织中PCDH8启动子甲基化状态可能有助于识别需要积极术后干预以改善预后的患者。
