评估不同膳食抗氧化剂对肾细胞癌中过氧化氢的抗凋亡活性。
Evaluation of anti-apoptotic activity of different dietary antioxidants in renal cell carcinoma against hydrogen peroxide.
作者信息
Garg Neeraj K, Mangal Sharad, Sahu Tejram, Mehta Abhinav, Vyas Suresh P, Tyagi Rajeev K
机构信息
Department of Pharmaceutical Sciences, Dr. H. S. Gour University, Sagar, (M.P.), India.
出版信息
Asian Pac J Trop Biomed. 2011 Jan;1(1):57-63. doi: 10.1016/S2221-1691(11)60069-5.
OBJECTIVE
To evaluate the anti-apoptotic and radical scavenging activities of dietary phenolics, namely ascorbic acid,α-tocopherol acetate, citric acid, salicylic acid, and estimate H2O2-induced apoptosis in renal cell carcinoma cells.
METHODS
The intracellular antioxidant potency of antioxidants was investigated. H2O2-induced apoptosis in RCC-26 was assayed with the following parameters: cell viability (% apoptosis), nucleosomal damage and DNA fragmentation, bcl-2 levels and flow cytometery analysis (ROS production evaluation).
RESULTS
The anticancer properties of antioxidants such as ascorbic acid, α-tocopherol acetate, citric acid, salicylic acid with perdurable responses were investigated. It was observed that these antioxidants had protective effect (anti-apoptotic activity) against hydrogen peroxide (H2O2) in renal cell carcinoma (RCC-26) cell line.
CONCLUSIONS
This study reveals and proves the anticancer properties. However, in cancer cell lines anti-apoptotic activity can indirectly reflect the cancer promoter activity through radicals scavenging, and significantly protect nucleus and bcl-2.
目的
评估膳食酚类物质（即抗坏血酸、α-生育酚醋酸酯、柠檬酸、水杨酸）的抗凋亡和自由基清除活性，并评估过氧化氢诱导肾癌细胞凋亡的情况。
方法
研究了抗氧化剂的细胞内抗氧化能力。用以下参数检测过氧化氢诱导RCC - 26细胞凋亡的情况：细胞活力（凋亡百分比）、核小体损伤和DNA片段化、bcl - 2水平以及流式细胞术分析（活性氧生成评估）。
结果
研究了抗坏血酸、α-生育酚醋酸酯、柠檬酸、水杨酸等抗氧化剂的抗癌特性及其持久反应。观察到这些抗氧化剂对肾癌细胞系（RCC - 26）中的过氧化氢（H2O2）具有保护作用（抗凋亡活性）。
结论
本研究揭示并证明了这些抗氧化剂的抗癌特性。然而，在癌细胞系中，抗凋亡活性可通过自由基清除间接反映癌症促进活性，并显著保护细胞核和bcl - 2。
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