El-Sayyad Hassan I, Sakr Saber A, Badawy Gamal M, Afify Hanaa S
Department of Zoology, Faculty of Science, Mansoura University, Mansoura, Egypt.
Asian Pac J Trop Biomed. 2011 Aug;1(4):253-60. doi: 10.1016/S2221-1691(11)60038-5.
To evaluate the hazardous effects of fried potato chips upon the retina of two developmental stages of the albino rats aged 7 and 14 days from parturition.
PREGNANT RATS WERE ARRANGED INTO TWO GROUPS: control pregnant rats and consequently their delivered newborns until reaching 7 and 14 days old from parturition and fried potato chips group in which pregnant rats at the 6th day of gestation maintained on diet formed of fried potato chips supplied from the market mixed with standard diet at a concentration of 50% per each till 7 and 14 post-partum. Three fold integrated approaches were adopted, namely, histological, ultrastructural and proteomic analysis.
Histological examination of the retina of the experimental offsprings revealed many histopathological changes, including massive degeneration, vacuolization and cell loss in the ganglion cell layer, as well as general reduction in retinal size. At the ultrastructural level, the retina of experimental offsprings exhibited number of deformities, including ill differentiated and degenerated nuclear layer, malformed and vacuolated pigment epithelium with vesiculated and fragmented rough endoplasmic reticulum, degenerated outer segment of photoreceptors, as well as swollen choriocapillaris and loss of neuronal cells. Proteomic analysis of retina of the two experimental developmental stages showed variations in the expressed proteins as a result of intoxication which illustrated the adverse toxic effects of fried potato chips upon the retina.
It can be concluded that the effect of fried potato chips on the development of retina in rats may be due to the presence of acrylamide or its metabolite.
评估油炸薯片对出生后7天和14天两个发育阶段的白化大鼠视网膜的有害影响。
将怀孕大鼠分为两组：对照怀孕大鼠及其分娩的新生大鼠，直至出生后7天和14天；油炸薯片组，其中妊娠第6天的怀孕大鼠食用由市售油炸薯片与标准饮食按50%的浓度混合而成的饮食，直至产后7天和14天。采用了三种综合方法，即组织学、超微结构和蛋白质组学分析。
对实验后代视网膜的组织学检查发现许多组织病理学变化，包括神经节细胞层的大量变性、空泡化和细胞丢失，以及视网膜大小普遍减小。在超微结构水平上，实验后代的视网膜表现出许多畸形，包括核层分化不良和退化、色素上皮畸形和空泡化，伴有内质网囊泡化和碎片化、光感受器外段退化，以及脉络膜毛细血管肿胀和神经元细胞丢失。对两个实验发育阶段视网膜的蛋白质组学分析表明，中毒导致表达的蛋白质发生变化，这说明了油炸薯片对视网膜的不良毒性作用。
可以得出结论，油炸薯片对大鼠视网膜发育的影响可能是由于丙烯酰胺或其代谢产物的存在。
