Mirzaei A, Sharifiyazdi H, Ahmadi M R, Ararooti T, Ghasrodashti A Rowshan, Kadivar A
Department of Clinical Science, School of Veterinary Medicine, Shiraz University, Shiraz 71345, Iran.
Asian Pac J Trop Biomed. 2012 Oct;2(10):765-9. doi: 10.1016/S2221-1691(12)60226-3.
To investigate the relationship between polymorphism within the 5'-untranslated region (5'-UTR) of IGF-I gene and its periparturient concentration in Iranian Holstein dairy cows.
Blood samples (5 mL, n = 37) were collected by caudal venipuncture from each animal into sample tubes containing the EDTA and DNA was extracted from blood. In order to measure IGF-I concentration the collection of blood samples (n = 111) was also done at 14 d before calving (prepartum), 25 and 45 d postpartum.
We found evidence for a significant effect of C to T mutation in position 512 of IGF-I gene on its serum concentration in dairy cows in Iran. Cows with CC genotype had significantly higher concentration (Mean±SD) of IGF-I at 14 d prepartum (91.8±18.1) µg/L compared to those with TT genotype (73.3±14.4) µg/L (P=0.04). A significant trend (quadratic) was found for IGF-I concentration, as higher in CC cows compared to ones with TT genotype, during the 14 d before calving to 45 d postpartum (P=0.01).
We concluded that C/T transition in the promoter region of IGF-I gene can influence the serum concentration of IGF-I in periparturient dairy cows.
研究伊朗荷斯坦奶牛胰岛素样生长因子-I（IGF-I）基因5'-非翻译区（5'-UTR）多态性与其围产期浓度之间的关系。
通过尾静脉穿刺从每头动物采集5 mL血液样本（n = 37），放入含有乙二胺四乙酸（EDTA）的样本管中，并从血液中提取DNA。为了测量IGF-I浓度，还在产犊前14天（产前）、产后25天和45天采集血液样本（n = 111）。
我们发现IGF-I基因第512位C到T突变对伊朗奶牛血清浓度有显著影响的证据。CC基因型奶牛在产前14天的IGF-I浓度（均值±标准差）显著高于TT基因型奶牛，分别为（91.8±18.1）μg/L和（73.3±14.4）μg/L（P = 0.04）。在产犊前14天至产后45天期间，发现IGF-I浓度存在显著趋势（二次曲线），CC基因型奶牛的浓度高于TT基因型奶牛（P = 0.01）。
我们得出结论，IGF-I基因启动子区域的C/T转换可影响围产期奶牛血清中IGF-I的浓度。
