Tucker D C, Gautier C H
Department of Psychology, University of Alabama, Birmingham 35294.
Ann N Y Acad Sci. 1990;588:120-30. doi: 10.1111/j.1749-6632.1990.tb13202.x.
Embryonic heart cultured in oculo differentiates into adult-like heart tissue, increases in size, and maintains spontaneous activity driven by a defined pacemaker. The growth and beating rate of embryonic heart maturing in oculo are modulated by its neural milieu. Culture in a sympathetically denervated eye chamber compromised growth and increased intrinsic beating rate. Exposure to an additional source of sympathetic neurons increased parasympathetic control of heart rate but did not alter growth or intrinsic beating rate. Because eye chamber sympathectomy alters neurotransmitter expression in parasympathetic and sensory neurons, it is possible that the growth inhibition observed in heart grafts is not a direct consequence of preventing sympathetic innervation. This possibility is being tested in additional experiments.
在眼内培养的胚胎心脏可分化为类似成体的心脏组织，体积增大，并由特定起搏器驱动维持自发活动。眼内成熟的胚胎心脏的生长和跳动速率受其神经环境调节。在交感神经去神经支配的眼房内培养会损害生长并增加固有跳动速率。暴露于额外的交感神经元来源会增加副交感神经对心率的控制，但不会改变生长或固有跳动速率。由于眼房交感神经切除术会改变副交感神经和感觉神经元中的神经递质表达，因此心脏移植物中观察到的生长抑制可能不是阻止交感神经支配的直接后果。这种可能性正在其他实验中进行测试。
