Role of sympathetic innervation in cardiac development in oculo.

Embryonic heart cultured in oculo differentiates into adult-like heart tissue, increases in size, and maintains spontaneous activity driven by a defined pacemaker. The growth and beating rate of embryonic heart maturing in oculo are modulated by its neural milieu. Culture in a sympathetically denervated eye chamber compromised growth and increased intrinsic beating rate. Exposure to an additional source of sympathetic neurons increased parasympathetic control of heart rate but did not alter growth or intrinsic beating rate. Because eye chamber sympathectomy alters neurotransmitter expression in parasympathetic and sensory neurons, it is possible that the growth inhibition observed in heart grafts is not a direct consequence of preventing sympathetic innervation. This possibility is being tested in additional experiments.