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在体外微繁殖 Polygonatum verticillatum（L.）All.，印度北部一种重要的濒危药用草本植物。

In vitro micropropagation in Polygonatum verticillatum (L.) All. an important threatened medicinal herb of Northern India.

机构信息

Department of Botany, Hemwati Nandan Bahuguna Garhwal University (A Central University), Campus Pauri (Pauri Garhwal), 246001 Uttarakhand, India.

出版信息

Physiol Mol Biol Plants. 2012 Jan;18(1):89-93. doi: 10.1007/s12298-011-0091-5. Epub 2011 Dec 10.

DOI:10.1007/s12298-011-0091-5
PMID:23573044
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3550528/
Abstract

An ideal micropropagation method of Polygonatum verticillatum has been developed using stem disc explants. Multiple shoots were initiated from stem disc explants on Murashige and Skoog (MS) medium fortified with different concentrations (0.25-10.0 mgl(-1)) and combinations of cytokinins (BAP, Kn and TDZ) along with (0.5-1.0 mgl(-1)) auxins (NAA/IBA/IAA). 1.0 mgl(-1) BAP with 0.5 mgl(-1) NAA was found to be the most effective in producing maximum number of shoots. Regular subculturing of these in vitro multiple shoots induced profuse growth of lateral roots in the same medium. Individual shoots were excised and rooted in vitro on half strength MS medium with 1.0 mgl(-1) NAA. Regenerants were hardened in growth chamber with high humidity and showed a high rate of survival.

摘要

已经开发出一种利用茎盘外植体的黄精属植物理想的微繁殖方法。在附加不同浓度（0.25-10.0 mgl(-1))和细胞分裂素（BAP、Kn 和 TDZ）组合（0.5-1.0 mgl(-1))的 Murashige 和 Skoog (MS) 培养基上，从茎盘外植体上诱导出多个芽。发现 1.0 mgl(-1) BAP 与 0.5 mgl(-1) NAA 的组合最有效地产生最多数量的芽。在相同的培养基中，对这些离体多芽进行常规继代培养，诱导大量侧根生长。将单个芽切下并在含有 1.0 mgl(-1) NAA 的半强度 MS 培养基上进行离体生根。再生体在高湿度的生长室中硬化，并表现出高成活率。

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本文引用的文献

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