Plant Biotechnology Laboratory, Department of Botany, JNV University, 342001, Jodhpur, India.
Plant Cell Rep. 1995 Dec;15(3-4):278-81. doi: 10.1007/BF00193736.
A method for micropropagation of mature trees of Capparis decidua was developed. Multiple shoots were obtained from nodal explants on Murashige and Skoog's (1962) medium+0.1mgl(-1) NAA+5.0mgl(-1)BAP+additives (50mgl(-1) ascorbic acid and25 mgl(-1) each of adenine sulphate, L-arginine and citric acid) at 28 ± 2°C, 12 h/dphotoperiod and 35-40 μmol m(-2)s(-1) photon flux density. The shoots were multiplied by (i) subculture of nodal shoot segments onto MS +0.1 mgl(--1) IAA+1.0mgl(-1) BAPH+additives, and (ii) repeated transfer of original explant onto MS+ 0.1mgl(-1) IAA+mg l(-1) BAP+additives, at intervals of 3 weeks. Sixty to 70% of the shoots rooted when pulse treated with 100 mg l(-1) IBA in half strength MS liquid medium for 4h, and then transferred onto hormone-free half-strength agar-gelled MS basal saltmedium. Incubation in dark at 33 ± 2°C for 6d favoured root induction. In vitro hardened plants were transferred to pots.
开发了一种用于繁殖 Capparis decidua 成熟树木的微繁殖方法。在 28±2°C、12 h/d 光周期和 35-40 μmol m(-2)s(-1) 光通量密度的条件下，从节间外植体在 Murashige 和 Skoog（1962）培养基+0.1mgl(-1) NAA+5.0mgl(-1)BAP+添加剂（50mgl(-1) 抗坏血酸和 25 mgl(-1) 硫酸腺嘌呤、L-精氨酸和柠檬酸各）上获得了多个芽。通过（i）将节间芽段接种到 MS+0.1 mgl(--1) IAA+1.0mgl(-1) BAPH+添加剂上，以及（ii）将原始外植体每隔 3 周重复转移到 MS+0.1mgl(-1) IAA+mg l(-1) BAP+添加剂上，使芽进行增殖。当用半强度 MS 液体培养基中的 100mg l(-1) IBA 脉冲处理 4h 后，60-70%的芽生根，然后转移到无激素的半强度琼脂凝胶 MS 基本盐培养基上。在黑暗中于 33±2°C 下培养 6d 有利于诱导生根。在体外硬化的植物被转移到盆中。
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