Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
Evid Based Complement Alternat Med. 2013;2013:780504. doi: 10.1155/2013/780504. Epub 2013 Mar 12.
In this study, the effects of Chlorella vulgaris (CV) on replicative senescence of human diploid fibroblasts (HDFs) were investigated. Hot water extract of CV was used to treat HDFs at passages 6, 15, and 30 which represent young, presenescence, and senescence ages, respectively. The level of DNA damage was determined by comet assay while apoptosis and cell cycle profile were determined using FACSCalibur flow cytometer. Our results showed direct correlation between increased levels of damaged DNA and apoptosis with senescence in untreated HDFs (P < 0.05). Cell cycle profile showed increased population of untreated senescent cells that enter G0/G1 phase while the cell population in S phase decreased significantly (P < 0.05). Treatment with CV however caused a significant reduction in the level of damaged DNA and apoptosis in all age groups of HDFs (P < 0.05). Cell cycle analysis showed that treatment with CV increased significantly the percentage of senescent HDFs in S phase and G2/M phases but decreased the population of cells in G0/G1 phase (P < 0.05). In conclusion, hot water extract of Chlorella vulgaris effectively decreased the biomarkers of ageing, indicating its potential as an antiageing compound.
在这项研究中，研究了小球藻（CV）对人二倍体成纤维细胞（HDFs）复制性衰老的影响。使用 CV 的热水提取物分别在传代 6、15 和 30 时处理 HDFs，分别代表年轻、衰老前期和衰老期。彗星试验测定 DNA 损伤水平，流式细胞仪测定细胞凋亡和细胞周期。结果显示，未处理的 HDFs 中，DNA 损伤和凋亡水平与衰老呈正相关（P<0.05）。细胞周期分析显示，未处理的衰老细胞进入 G0/G1 期的细胞群体增加，而 S 期细胞群体显著减少（P<0.05）。然而，用 CV 处理导致所有年龄组的 HDFs 的 DNA 损伤和凋亡水平显著降低（P<0.05）。细胞周期分析显示，CV 处理显著增加了 S 期和 G2/M 期衰老 HDFs 的百分比，但降低了 G0/G1 期细胞的数量（P<0.05）。综上所述，小球藻的热水提取物可有效降低衰老生物标志物，表明其具有抗衰老作用。
