影响印迹在尼龙或硝酸纤维素膜上的核酸杂交的参数。
Parameters affecting hybridization of nucleic acids blotted onto nylon or nitrocellulose membranes.
作者信息
Twomey T A, Krawetz S A
机构信息
Department of Molecular Biology and Genetics, Wayne State University, Detroit, MI 48201.
出版信息
Biotechniques. 1990 May;8(5):478-82.
PMID:2357366
Abstract
Nine different nylon and nitrocellulose membranes were compared utilizing four different methods of attaching the nucleic acid target. Nylon membranes repeatedly demonstrated increased sensitivity as compared to nitrocellulose membranes. Sensitivity could also be enhanced by mildly denaturing the target prior to attachment onto the membrane. This was achieved by either UV cross-linking or baking.
摘要
使用四种不同的核酸靶标固定方法，对九种不同的尼龙膜和硝酸纤维素膜进行了比较。与硝酸纤维素膜相比，尼龙膜反复显示出更高的灵敏度。在将靶标固定到膜上之前，通过轻度变性靶标也可以提高灵敏度。这可以通过紫外线交联或烘烤来实现。
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