Gross R H
Dartmouth College, Hanover, NH 03755.
Biotechniques. 1990 Jun;8(6):684-9.
The Gene Construction Kit is a new tool for manipulating and displaying DNA sequence information. Constructs can be displayed either graphically or as formatted sequence. Segments of DNA can be cut out with restriction enzymes and pasted into other sites. The program keeps track of staggered ends and notifies the user of incompatibilities and offers a choice of ligation options. Each segment of a construct can have its own defined thickness, pattern, direction and color. The sequence listing can be displayed in any font and style in user defined grouping. Nucleotide positions can be displayed as can restriction sites and protein sequences. The DNA can be displayed as either single- or double-stranded. Restriction sites can be readily marked. Alternative views of the DNA can be maintained and the history of the construct automatically stored. Gel electrophoresis patterns can be generated and can be used in cloning project design. Extensive comments can be stored with the construct and can be searched rapidly for key words. High quality illustrations showing multiple editable constructs with added graphics and text information can be generated for slides, posters or publication.
基因构建工具包是一种用于处理和展示DNA序列信息的新工具。构建体可以以图形方式或格式化序列的形式展示。DNA片段可以用限制性内切酶切割下来并粘贴到其他位点。该程序会跟踪交错末端，通知用户不兼容性，并提供连接选项供选择。构建体的每个片段都可以有其自己定义的厚度、模式、方向和颜色。序列列表可以以用户定义的分组方式以任何字体和样式显示。核苷酸位置、限制性酶切位点和蛋白质序列都可以显示。DNA可以显示为单链或双链。限制性酶切位点可以很容易地标记出来。可以保留DNA的替代视图，并自动存储构建体的历史记录。可以生成凝胶电泳图谱，并可用于克隆项目设计。可以将大量注释与构建体一起存储，并可快速搜索关键词。可以生成高质量的插图，展示带有添加的图形和文本信息的多个可编辑构建体，用于幻灯片、海报或出版物。
