Department of Chemistry and Biomolecular Sciences and ‡Australian Proteome Analysis Facility (APAF), Research Park Drive, Macquarie University , Sydney, 2109 NSW, Australia.
J Agric Food Chem. 2013 May 1;61(17):4079-88. doi: 10.1021/jf305276c. Epub 2013 Apr 19.
Chicken eggs are recognized for their versatility as a food product and as a model for research in biology and medicine. This study investigated the egg white as a source of bioactive compounds. Egg white was fractionated using tandem ion exchange chromatography (SAX and SCX), and seven fractions were assessed for any associated bioactivity. Four fractions at various protein concentrations were shown to contain proliferative bioactivity that exceeded the FBS control. The most potent fraction (6) was used in an in vitro wound closure assay to demonstrate a positive influence on cell migration and restored scratch wounds more rapidly than the control. LC-MS/MS identified 33 proteins in fraction 6 of egg white, most of which play important roles in cell growth and development, signaling, motility, and proliferation. These candidate bioactives suggest that the egg white contains essential compounds that contribute to the growth of an embryo prior to fertilization.
鸡蛋因其作为食品的多功能性以及在生物学和医学研究中的模型而受到认可。本研究探讨了蛋清作为生物活性化合物的来源。蛋清使用串联离子交换色谱（SAX 和 SCX）进行分级，评估了七个级分是否具有任何相关的生物活性。四个不同蛋白质浓度的级分显示出具有超过 FBS 对照的增殖生物活性。最有效的级分（6）用于体外伤口闭合测定，以证明对细胞迁移的积极影响，并比对照更快地修复划痕伤口。LC-MS/MS 在蛋清的第 6 级分中鉴定出 33 种蛋白质，其中大多数在细胞生长和发育、信号转导、运动和增殖中发挥重要作用。这些候选生物活性表明蛋清中含有对受精前胚胎生长至关重要的化合物。
