Stangegaard Michael, Børsting Claus, Ferrero-Miliani Laura, Frank-Hansen Rune, Poulsen Lena, Hansen Anders J, Morling Niels
1University of Copenhagen, Faculty of Health Sciences, Copenhagen, Denmark.
J Lab Autom. 2013 Oct;18(5):404-10. doi: 10.1177/2211068213484472. Epub 2013 Apr 10.
Extraction of DNA using magnetic bead-based techniques on automated DNA extraction instruments provides a fast, reliable, and reproducible method for DNA extraction from various matrices. Here, we have compared the yield and quality of DNA extracted from FTA cards using four automated extraction protocols on three different instruments. The extraction processes were repeated up to six times with the same pieces of FTA cards. The sample material on the FTA cards was either blood or buccal cells. With the QIAamp DNA Investigator and QIAsymphony DNA Investigator kits, it was possible to extract DNA from the FTA cards in all six rounds of extractions in sufficient amount and quality to obtain complete short tandem repeat (STR) profiles on a QIAcube and a QIAsymphony SP. With the PrepFiler Express kit, almost all the extractable DNA was extracted in the first two rounds of extractions. Furthermore, we demonstrated that it was possible to successfully extract sufficient DNA for STR profiling from previously processed FTA card pieces that had been stored at 4 °C for up to 1 year. This showed that rare or precious FTA card samples may be saved for future analyses even though some DNA was already extracted from the FTA cards.
在自动化DNA提取仪器上使用基于磁珠的技术提取DNA，为从各种基质中提取DNA提供了一种快速、可靠且可重复的方法。在此，我们比较了在三种不同仪器上使用四种自动化提取方案从FTA卡中提取的DNA产量和质量。使用相同的FTA卡片段将提取过程重复多达六次。FTA卡上的样本材料为血液或颊细胞。使用QIAamp DNA Investigator和QIAsymphony DNA Investigator试剂盒，在所有六轮提取中都能够从FTA卡中提取出足量且高质量的DNA，以便在QIAcube和QIAsymphony SP上获得完整的短串联重复序列（STR）图谱。使用PrepFiler Express试剂盒时，几乎所有可提取的DNA在前两轮提取中就被提取出来了。此外，我们证明了从先前处理过且已在4°C下保存长达1年的FTA卡片段中成功提取足够用于STR分析的DNA是可行的。这表明即使已经从FTA卡中提取了一些DNA，珍稀或珍贵的FTA卡样本仍可留存以备将来分析。
