State Key Laboratory of Surface Physics and Department of Physics and Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Fudan University, 220, Handan Road, Shanghai 200433, China.
Phys Chem Chem Phys. 2013 Oct 14;15(38):15727-33. doi: 10.1039/c3cp44227f. Epub 2013 Apr 11.
Gold nanorods (AuNRs) were conjugated with chlorin e6 (Ce6), a commonly used photosensitizer, to form AuNRs-Ce6 by electrostatic binding. Due to the strong surface plasmon resonance coupling, the fluorescence of conjugated Ce6 was enhanced 3-fold and the production of singlet oxygen was increased 1.4-fold. AuNRs-Ce6 were taken up by the HeLa and KB cell lines more easily than free Ce6, enhancing the intracellular delivery of Ce6. The increased cellular amount of Ce6 leads to a 3-fold more efficient photodynamic killing of these two cell lines. This demonstrates the potential of this approach to improve photodynamic detection and therapy of cancers.
金纳米棒（AuNRs）通过静电结合与叶绿素 e6（Ce6）连接，形成 AuNRs-Ce6。由于强烈的表面等离子体共振耦合，共轭 Ce6 的荧光增强了 3 倍，单线态氧的生成增加了 1.4 倍。与游离的 Ce6 相比，AuNRs-Ce6 更容易被 HeLa 和 KB 细胞系摄取，增强了 Ce6 的细胞内传递。细胞内 Ce6 含量的增加导致这两种细胞系的光动力杀伤效率提高了 3 倍。这证明了这种方法在提高癌症的光动力检测和治疗方面的潜力。
