Mitrakul K, Vongsavan K, Suratanachaikul P
Department of Pediatric Dentistry, Faculty of Dentistry, Mahidol University, 6 Yothee Street, Rajthevee, Phaya Thai, Bangkok, 10400, Thailand,
Eur Arch Paediatr Dent. 2013 Apr;14(2):83-7. doi: 10.1007/s40368-013-0017-8. Epub 2013 Apr 11.
To detect the prevalence of Streptococcus mutans and Lactobacillus fermentum in plaque obtained from severe early childhood caries (S-ECC) and caries-free (CF) groups and to assess any association between their prevalence, feeding habits and oral hygiene practices within each group.
Sixty-four supra-gingival plaque samples were collected from S-ECC and CF groups (CF = 30, S-ECC = 34). All participants were asked about feeding habits and oral hygiene care behaviour by direct questionnaire.
After DNA extraction, S. mutans and L. fermentum were amplified using standard PCR. Nested PCR was performed as a second identification method for S. mutans.
Mean age in CF and S-ECC was 2.29 ± 0.42 and 2.49 ± 0.41 years, respectively. Mean dmft in S-ECC was 4.51 ± 0.72. By standard PCR, no S. mutans was detected in CF group, while 10 % was detected in S-ECC group. By nested PCR, S. mutans was detected for 48 and 50 % in CF and S-ECC, respectively. L. fermentum was detected for 16 and 40 % in CF and S-ECC, respectively.
Nested PCR gave a higher detection frequency than standard PCR but not significant (p ≤ 0.05). The prevalence of S. mutans was not different between the two groups, while the prevalence of L. fermentum was significantly associated with S-ECC (p ≤ 0.05).
检测重度幼儿早期龋(S-ECC)组和无龋(CF)组牙菌斑中变形链球菌和发酵乳杆菌的流行情况,并评估每组中它们的流行率、喂养习惯和口腔卫生习惯之间的关联。
从S-ECC组和CF组收集64份龈上菌斑样本(CF组 = 30份,S-ECC组 = 34份)。通过直接问卷调查询问所有参与者的喂养习惯和口腔卫生护理行为。
DNA提取后,使用标准PCR扩增变形链球菌和发酵乳杆菌。巢式PCR作为变形链球菌的第二种鉴定方法。
CF组和S-ECC组的平均年龄分别为2.29±0.42岁和2.49±0.41岁。S-ECC组的平均乳牙龋失补牙数(dmft)为4.51±0.72。通过标准PCR,CF组未检测到变形链球菌,而S-ECC组检测到10%。通过巢式PCR,CF组和S-ECC组中变形链球菌的检测率分别为48%和50%。CF组和S-ECC组中发酵乳杆菌的检测率分别为16%和40%。
巢式PCR的检测频率高于标准PCR,但差异无统计学意义(p≤0.05)。两组间变形链球菌的流行率无差异,而发酵乳杆菌的流行率与S-ECC显著相关(p≤0.05)。