Kus J
Department of Tuberculosis and Pulmonary Diseases, Institute of Tuberculosis and Chest Diseases, Warsaw, Poland.
Clin Exp Allergy. 1990 Mar;20(2):165-70. doi: 10.1111/j.1365-2222.1990.tb02662.x.
Peripheral blood mononuclear cell (MNC) response to house dust (HD) stimulation in cultures was studied in a group of 35 subjects with asthma and a multiple positive skin test (ST) reaction to inhalant allergens including HD, and in 19 healthy controls. The MNC response to allergen stimulation was assessed by interleukin-2 receptor (IL-2R) expression identified with an anti-Tac monoclonal antibody. Lymphocyte subpopulations of baseline and cultured cells were also analysed. The percentage of IL-2R presenting cells increased significantly in HD-driven cultures in the asthma group compared to controls. The increase in proportion of IL-2R+ cells was closely related to the increase in CD4+ percentage of cultured cells and was accompanied by a decrease in proportion of CD8+ lymphocytes and monocytes/macrophages, suggesting that HD-activated cells were CD4+ lymphocytes. Lymphocyte activation, measured by IL-2R expression, was significantly higher in the group of asthmatics, and positive dual (early and late) skin reaction to HD, as compared to those with single early reaction.
在一组35名患有哮喘且对包括屋尘（HD）在内的吸入性过敏原多项皮肤试验（ST）呈阳性反应的受试者以及19名健康对照者中，研究了外周血单个核细胞（MNC）在培养物中对屋尘（HD）刺激的反应。通过用抗Tac单克隆抗体鉴定的白细胞介素-2受体（IL-2R）表达来评估MNC对过敏原刺激的反应。还分析了基线细胞和培养细胞的淋巴细胞亚群。与对照组相比，哮喘组中HD驱动培养物中呈现IL-2R的细胞百分比显著增加。IL-2R +细胞比例的增加与培养细胞中CD4 +百分比的增加密切相关，并伴有CD8 +淋巴细胞和单核细胞/巨噬细胞比例的降低，这表明HD激活的细胞是CD4 +淋巴细胞。与单次早期反应者相比，通过IL-2R表达测量的淋巴细胞活化在哮喘组以及对HD呈阳性双重（早期和晚期）皮肤反应者中显著更高。
