Programa de Pós-Graduação em Ciências Farmacêuticas, Department of Pharmacy, Universidade Estadual de Maringá, Maringá, PR, Brazil.
J Sep Sci. 2013 Apr;36(7):1247-54. doi: 10.1002/jssc.201200911.
The phenolic compounds are the main phytochemical constituents of the bark of Trichilia catigua and are commonly used for medicinal purposes. An HPLC method for the simultaneous quantification of phenolic compounds (procyanidin B2 (PB2), epicatechin (EPC), chinchonains Ia, Ib, IIa, IIb, catechin, and chrologenic acid) in T. catigua extract was developed and validated. A suitable chromatographic system was selected, which uses a gradient elution with methanol/ACN (75:25), and water both with 0.05% TFA, as mobile phase, column Luna, 280 nm, and flow 0.4 mL/min. Validation of the analytical method was based on the parameters: linearity, precision, LODs and LOQs, accuracy, robustness, and stability. The method showed linearity for PB2 and EPC, in the range 10-120 μg/mL with good correlation coefficients (>0.996). For precision, the repeatability ranged from 1.89 to 3.23%, and the values for accuracy for PB2 and EPC were 95 and 89%, respectively. The LODs and LOQs for PB2 were 1.36 and 4.12 μg/mL, and for EPC were 2.18 and 6.61 μg/mL, respectively. The method was robust under the conditions employed. The proposed method could be employed for quality assessment of T. catigua, as well as pharmaceutical products.
酚类化合物是 Trichilia catigua 树皮中的主要植物化学成分，常用于药用。本研究建立并验证了一种高效液相色谱法（HPLC），用于同时定量分析 T. catigua 提取物中的酚类化合物（原花青素 B2（PB2）、表儿茶素（EPC）、金鸡纳因 Ia、Ib、IIa、IIb、儿茶素和绿原酸）。选择了合适的色谱系统，采用甲醇/乙腈（75:25）和水作为流动相，均含有 0.05%TFA，梯度洗脱，柱温为 280nm，流速为 0.4mL/min。分析方法的验证基于线性、精密度、检出限和定量限、准确度、稳健性和稳定性等参数。该方法对 PB2 和 EPC 的线性范围为 10-120μg/mL，相关性良好（>0.996）。精密度方面，重复性范围为 1.89-3.23%，PB2 和 EPC 的准确度值分别为 95%和 89%。PB2 的检出限和定量限分别为 1.36μg/mL 和 4.12μg/mL，EPC 的检出限和定量限分别为 2.18μg/mL 和 6.61μg/mL。该方法在所用条件下稳健。该方法可用于 T. catigua 的质量评估以及药物产品的质量评估。
