Rao P S, Cavanagh D M, Fiorica J V, Spaziani E
Department of Obstetrics and Gynecology, University of South Florida College of Medicine, Tampa 33612-4799.
Circ Shock. 1990 Jul;31(3):333-42.
This study was designed to evaluate the effects of endotoxin infusion (0.25 mg/kg) over a 4-hr period on renal function and tubular enzyme activity. Endotoxin administration resulted in a decrease in blood pressure, osmolar clearance, and creatinine clearance (P less than 0.05). The enzyme activities of alkaline phosphatase (ALP) and lactic dehydrogenase (LDH) in the urine increased, as did the serum creatinine (P less than 0.05). There were no significant changes in the renal artery flow, urinary output, heart rate, serum electrolytes, and serum enzyme activities. In contrast, in the saline control group, the renal artery flow increased (P less than 0.05), whereas the serum creatinine, urinary ALP, and urinary LDH decreased over time. All other parameters remained relatively stable. These data suggest that an increase in urinary enzyme activity reflects compromised renal function and is independent of the renal artery flow. This may have clinical application in detecting early renal damage due to endotoxemia or sepsis.
本研究旨在评估在4小时内输注内毒素（0.25毫克/千克）对肾功能和肾小管酶活性的影响。给予内毒素导致血压、渗透清除率和肌酐清除率降低（P<0.05）。尿中碱性磷酸酶（ALP）和乳酸脱氢酶（LDH）的酶活性增加，血清肌酐也增加（P<0.05）。肾动脉血流量、尿量、心率、血清电解质和血清酶活性无显著变化。相比之下，在生理盐水对照组中，肾动脉血流量增加（P<0.05），而血清肌酐、尿ALP和尿LDH随时间下降。所有其他参数保持相对稳定。这些数据表明，尿酶活性增加反映了肾功能受损，且与肾动脉血流量无关。这可能在检测内毒素血症或脓毒症引起的早期肾损伤方面具有临床应用价值。
