Luo Guang-heng, Luo Lei, Yang Xiu-shu, Liu Jun, Sun Zhao-lin, Xia Shu-jie
Department of Urology, Guizhou Provincial People's Hospital, Guiyang 550002, China.
Zhonghua Yi Xue Za Zhi. 2013 Jan 22;93(4):261-4.
To study the re-epithelialization of dog's prostate urethra after two-micron laser resection of the prostate (TmLRP) and the role of CK34 (CK34βE12)-positive cells in this process.
TmLRP were performed in 15 elder male dogs and wound specimens harvested at Days 3, 7 and 14 respectively. Immunohistochemical staining was performed to determine the expression levels of CK34 and prostate specific antigen (PSA) in prostatic urethra urothelial cells.
There was no CK34-positive cells in surgical wounds after 3 days while a cluster of CK34-positive cells were found to cover surgical wounds after 7 days, and these cells attached to residual prostate tissues. At Day 14 post-operation, positive expression of CK34 was found in basal cells of newborn urothelium.
Prostate tissues are the important organizations for re-epithelialization of prostatic urethra after benign prostate hyperplasia surgery, and CK34-positive basal cells play an important role in this process.
研究犬前列腺尿道在经尿道双波长激光前列腺切除术（TmLRP）后的再上皮化过程以及细胞角蛋白34（CK34，即CK34βE12）阳性细胞在此过程中的作用。
对15只成年雄性犬实施TmLRP手术，并分别于术后第3天、第7天和第14天采集伤口标本。采用免疫组织化学染色法检测前列腺尿道尿路上皮细胞中CK34和前列腺特异性抗原（PSA）的表达水平。
术后3天时手术创面未见CK34阳性细胞，术后7天时可见一簇CK34阳性细胞覆盖手术创面，且这些细胞附着于残留的前列腺组织。术后第14天时，新生尿路上皮的基底细胞中出现CK34阳性表达。
前列腺组织是良性前列腺增生手术后前列腺尿道再上皮化的重要组织，CK34阳性基底细胞在此过程中发挥重要作用。
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