Department of Biology, Boston University, Boston, Massachusetts, USA.
Nat Protoc. 2013 May;8(5):916-23. doi: 10.1038/nprot.2013.044. Epub 2013 Apr 11.
Over the past 20 years, the starlet sea anemone, Nematostella vectensis, a small estuarine animal, has emerged as a powerful model system for field and laboratory studies of development, evolution, genomics, molecular biology and toxicology. Here we describe how to collect Nematostella, culture it through its entire sexual life cycle and induce regeneration for the production of clonal stocks. In less than 1 h at a suitable field site, a researcher on foot can collect hundreds of individual anemones. In a few months, it is possible to establish a laboratory colony that will be reliable in generating hundreds or thousands of fertilized eggs on a roughly weekly schedule. By inducing regeneration roughly every 2 weeks, in less than 6 months, one can establish a clonal stock consisting of hundreds of genetically identical anemones. These results can be achieved very inexpensively and without specialized equipment.
在过去的 20 年中，星状海葵（Nematostella vectensis）作为一种小型河口动物，已经成为用于发育、进化、基因组学、分子生物学和毒理学领域的现场和实验室研究的有力模式生物系统。在这里，我们将描述如何采集星状海葵、通过其整个有性生命周期进行培养以及诱导再生以产生克隆株。在合适的野外地点，步行的研究人员不到 1 小时就可以采集数百个个体海葵。在短短几个月内，就有可能建立一个实验室群体，每周可可靠地产生数百个或数千个受精卵。通过每两周左右诱导再生，不到 6 个月的时间内，就可以建立一个由数百个遗传上完全相同的海葵组成的克隆株。这些结果可以非常廉价地实现，而且不需要专门的设备。
