Department of Orthopedic Surgery, Navy General Hospital, Fucheng Road No. 6, Haidian District, Beijing, China.
J Orthop Res. 2013 Sep;31(9):1366-73. doi: 10.1002/jor.22369. Epub 2013 Apr 11.
We have previously explored the possibilities of allogenic intervertebral disc (IVD) curing disc degeneration disease in clinical practice. The results showed that the motion and stability of the spinal unit was preserved after transplantation of allogenic IVD in human beings at 5-year follow-up. However, mild degeneration was observed in the allogenic transplanted IVD cases. In this study, we construct the biological tissue engineering IVD by injecting the nucleus pulposus cells (NPCs) expressing human bone morphogenetic protein 7 (hBMP7) into cryopreserved IVD, and transplant the biological tissue engineering IVD into a beagle dog to investigate whether NPCs expressing hBMP7 could prevent the degeneration of the transplanted allogenic IVDs. At 24 weeks after transplantation, MRI scan showed that IVD allografts injected NPCs expressing hBMP7 have a slighter signs of degeneration than IVD allografts with NPCs or without NPCs. The range of motion of left-right rotation in the group without NPCs was bigger than that of two cells injection group. PKH-26-labeled cells were identified at IVD allograft. The study demonstrated that NPCs expressing hBMP7 could survive at least 24 weeks and prevent the degeneration of the transplanted IVD. This solution might have a potential role in preventing the IVD allograft degeneration in long time follow-up.
我们之前已经探讨了同种异体椎间盘（IVD）治疗临床椎间盘退行性疾病的可能性。结果表明，在 5 年随访中，同种异体 IVD 移植后脊柱单元的运动和稳定性得以保留。然而，在同种异体移植的 IVD 病例中观察到轻度退变。在这项研究中，我们通过将表达人骨形态发生蛋白 7（hBMP7）的核芯细胞（NPCs）注入冷冻保存的 IVD 中构建生物组织工程 IVD，并将生物组织工程 IVD 移植到比格犬体内，以研究表达 hBMP7 的 NPCs 是否可以防止同种异体 IVD 的退变。移植后 24 周，MRI 扫描显示，与 NPCs 或无 NPCs 注射的 IVD 同种异体移植物相比，注射表达 hBMP7 的 NPCs 的 IVD 同种异体移植物退变迹象较轻。无 NPCs 组的左右旋转活动范围大于两个细胞注射组。PKH-26 标记的细胞在 IVD 同种异体移植物中被识别。该研究表明，表达 hBMP7 的 NPCs 至少可以存活 24 周并防止移植 IVD 的退变。该解决方案可能在长期随访中预防 IVD 同种异体移植物退变方面具有潜在作用。
