Department of Clinical Dentistry - Centre for Clinical Dental Research, Faculty of Medicine and Dentistry, University of Bergen, PO Box 7804, N-5020 Bergen, Norway.
Arch Oral Biol. 2013 Jul;58(7):826-36. doi: 10.1016/j.archoralbio.2013.01.004. Epub 2013 Apr 10.
The aim of this study was to determine the effect of continuous compressive force (CF) on expression by human alveolar bone-derived osteoblasts (HOBs) of some specific molecules involved in bone remodelling.
HOBs were cultured with or without CF (control, 2.0, 4.0gcm(-2)) for 1, 3 and 7 days. Expression of alkaline phosphatase (ALP), type I collagen (Col I), osteopontin (OPN), osteocalcin (OCN), transcription factor Runx2, receptor activator of nuclear factor κB ligand (RANKL), osteoprotegerin (OPG) and prostaglandin E2 (PGE2) was analysed by real-time-polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA) and/or immunostaining.
The results revealed that CF upregulated ALP and Col I expression at both messenger RNA (mRNA) and protein levels but did not affect expression of OPN and OCN mRNA. Runx2 mRNA was inhibited by CF, which also altered the expression of molecules involved in osteoclastogenesis, by enhancing RANKL expression and suppressing OPG expression. At 4.0gcm(-2) of CF, the expression of RANKL and PGE2 was significantly upregulated.
The results suggest that initial application of CF on HOBs can simultaneously affect expression of markers related to both osteogenesis and osteoclastogenesis.
本研究旨在确定持续压力（CF）对人牙槽骨源性成骨细胞（HOB）表达某些参与骨重塑的特定分子的影响。
HOB 分别在有无 CF（对照，2.0、4.0gcm(-2))的条件下培养 1、3 和 7 天。通过实时聚合酶链反应（RT-PCR）、酶联免疫吸附试验（ELISA）和/或免疫染色分析碱性磷酸酶（ALP）、I 型胶原（Col I）、骨桥蛋白（OPN）、骨钙素（OCN）、转录因子 Runx2、核因子 κB 配体受体激活剂（RANKL）、骨保护素（OPG）和前列腺素 E2（PGE2）的表达。
结果表明，CF 在信使 RNA（mRNA）和蛋白水平上均上调 ALP 和 Col I 的表达，但不影响 OPN 和 OCN mRNA 的表达。CF 抑制 Runx2 mRNA，同时通过增强 RANKL 表达和抑制 OPG 表达来改变破骨细胞形成过程中涉及的分子的表达。在 4.0gcm(-2)的 CF 下，RANKL 和 PGE2 的表达显著上调。
结果表明，CF 对 HOB 的初始应用可以同时影响与成骨和破骨细胞形成相关的标志物的表达。
Zotero 插件